| 胭脂鱼微卫星富集文库的构建及其应用前景展望 |
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| 引用本文： | 杨钟,史芳,阙延福,熊美华,徐念,朱滨.胭脂鱼微卫星富集文库的构建及其应用前景展望[J].水生态学杂志,2009,30(2):107-112. |
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| 作者姓名： | 杨钟 史芳 阙延福 熊美华 徐念 朱滨 |
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| 作者单位： | 1. 水利部中国科学院水工程生态研究所,湖北,武汉,430079
2. 水利部中国科学院水工程生态研究所,湖北,武汉,430079;中国科学院水生生物研究所,湖北,武汉,430072 |
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| 基金项目： | 国家自然科学基金重大项目，武汉市青年科技晨光计划项目 |
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| 摘 要： | 以湖北武汉和四川宜宾人工增殖放流的胭脂鱼子一代酒精浸泡鳍条样本为材料,提取完整基因组DNA,选用人工合成的生物素标记(AAAG)7探针,采用FJASCO(fast isolation by AFLP of sequences containing repeats)磁珠富集法构建胭脂鱼(Myxocyprirnus asiaticus)微卫星富集文库.以Msel-N引物组和设计合成的微卫星核心序列引物(AAAG)5,用双引物PCR法筛选含有微卫星的阳性克隆,从54个阳性克隆中共获得22个微卫星序列,其中完美型(perfect)共15个(占68.2%),非完美型(imperfect)6个(占27.3%),混合型(compound)1个(占4.5%);(AAAG/ITYC)n序列在胭脂鱼的基因组DNA中含量非常丰富.根据微卫星侧翼序列最终设计并合成了18对胭脂鱼微卫星引物,经其有效性检验后,可应用于胭脂鱼遗传多样性、种群遗传结构等进一步研究及人工增殖放流效果评估.
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| 关 键 词： | 胭脂鱼 微卫星 富集文库 |
| 收稿时间： | 3/2/2009 12:00:00 AM |
Construction of Microsatellite Library Enriched and its Application Prospect in Chinese sucker(Myxocyprinus asiaticus) |
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| YANG Zhong,SHI Fang,QUE Yan-fu,XIONG Meihu,XU Nian and ZHU Bin.Construction of Microsatellite Library Enriched and its Application Prospect in Chinese sucker(Myxocyprinus asiaticus)[J].Journal of Hydroecology,2009,30(2):107-112. |
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| Authors: | YANG Zhong SHI Fang QUE Yan-fu XIONG Meihu XU Nian and ZHU Bin |
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| Institution: | Institute of Hydroecology ,Ministry of Water resources and Chinese Academy of Sciences, Wuhan 430079, China,Institute of Hydroecology ,Ministry of Water resources and Chinese Academy of Sciences, Wuhan 430079, China,Institute of Hydroecology ,Ministry of Water resources and Chinese Academy of Sciences, Wuhan 430079, China,Institute of Hydroecology ,Ministry of Water resources and Chinese Academy of Sciences, Wuhan 430079, China,Institute of Hydrobiology Chinese-Academy of Science, Wuhan 430071 China Institute of Hydroecology MWR & CAS, Wuhan 430079,China and Institute of Hydroecology ,Ministry of Water resources and Chinese Academy of Sciences, Wuhan 430079, China |
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| Abstract: | |
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| Keywords: | Chinese sucker microsatellite enriched library |
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