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大鳞副泥鳅7个群体遗传变异的微卫星分析
引用本文:游翠红,童金苟,俞小牧.大鳞副泥鳅7个群体遗传变异的微卫星分析[J].水生态学杂志,2012,33(1):84-91.
作者姓名:游翠红  童金苟  俞小牧
作者单位:1. 淡水生态与生物技术国家重点实验室,中国科学院水生生物研究所,湖北武汉430072;汕头大学海洋生物研究所,广东汕头515063
2. 淡水生态与生物技术国家重点实验室,中国科学院水生生物研究所,湖北武汉430072
基金项目:国家自然科学基金项目(30370225, 30271011),973项目(2010CB126305),FEBL课题(2008FBZ17)
摘    要:利用泥鳅的微卫星引物跨种扩增大鳞副泥鳅,通过家系扩增、PCR产物测序筛选出6个座位,用于大鳞副泥鳅7个野生群体的遗传结构分析。结果显示,群体的平均等位基因数在3.167与4.833之间,平均观测杂合度(Ho)在0.248与0.417之间,平均期望杂合度(He)在0.379与0.505之间,多个座位存在零等位基因、杂合子缺失现象,显示大鳞副泥鳅种群遗传多样性较低。采用 UPGMA 法对7个群体基于 DA 遗传距离进行聚类,可分为4类,位于长江中下游的沙市、澧县、武汉、鄱阳湖群体先聚为一类,后与石首群体聚类,最后与珠江水系的广州群体聚类,而位于长江上游的泸州群体则单独聚为一类。群体的 F-统计量(Fst)为0.2987,表明群体间存在显著的遗传分化,主要由泸州群体与其它地区群体间的遗传分化引起。

关 键 词:大鳞副泥鳅  微卫星  遗传多样性  种群遗传分化
收稿时间:2011/9/16 0:00:00
修稿时间:2011/11/6 0:00:00

Microsatellite DNA Analysis on Genetic Diversity of Seven Populations of Paramisgurnus dabryanus
YOU Cui hong,TONG Jin gou and YU Xiao mu.Microsatellite DNA Analysis on Genetic Diversity of Seven Populations of Paramisgurnus dabryanus[J].Journal of Hydroecology,2012,33(1):84-91.
Authors:YOU Cui hong  TONG Jin gou and YU Xiao mu
Institution:Marine Biology Institute of Shantou University,Institute of Hydrobiology, the Chinese Academy of Sciences,Institute of Hydrobiology, the Chinese Academy of Sciences
Abstract:By using the microsatellites of pond loach Misgurnus anguillcaudatus, cross-species PCR amplification and family segregation test were carried out in Paramisgurnus dabryanus, and six loci were proved to be suitable to analyze genetic diversity and population structure of the seven populations of P. dabryanus. The average number of alleles, observed heterozygosity (Ho) and expected heterozygosity (He) ranged from 3.167 to 4.833, 0.248 to 0.417 and 0.379 to 0.505, respectively. These results showed that the level of genetic diversity in P. dabryanus was relatively low. Null alleles and apparent deficiency of heterozygotes were detected at some loci. The cluster analysis by UPGMA method was carried out based on the DA genetic distance of seven populations. The results showed that all populations were pooled into four groups: Luzhou (upstream of the Yangtze River), Guangzhou (the Zhujiang water system), Shishou and other four populations (including Shashi, Lixian, Wuhan and Poyanghu, the middle and lower reaches of the Yangtze River). The value of the F-statistics was 0.2987 and this suggested that P. dabryanus exhibited a significant genetic differentiation, mostly resulting from genetic divergence between Luzhou and the other six populations.
Keywords:Paramisgurnus dabryanus  microsatellite  genetic diversity  population genetic differentiation
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