鳗弧菌感染对日本鑚部分免疫活性因子的影响

对日本蟳(Charybdis japonica)进行鳗弧菌(Vibrio anguillarum)悬液和生理盐水(对照)注射感染,研究其血清免疫因子超氧化物歧化酶(SOD)、酸性磷酸酶(ACP)、碱性磷酸酶(AKP)、过氧化氢酶(CAT)活性的变化,并对试验结果进行统计学分析。结果表明,注射鳗弧菌悬液组SOD活性12、24、48h均高于对照组,48h达到最高;CAT活性12、24h增加非常明显,12h达最高;AKP活性5、12、24h都有增加,均高于对照,24h最高;ACP活性12h开始上升,24h达最高。在人工感染鳗弧菌48h内,日本蟳体内的免疫因子发生了明显的变化,血清中SOD、ACP、AKP、CAT活性在不同时间段均有显著升高趋势,高于对照组水平。

Changes of Some Immunoactive Enzymes in Charybdis japonica Infected with Vibrio parahemolyticus

Charybdis japonica(A.Milne-Edwards) was infected with Vibrio anguillarum through injection,using normal saline as parallel control.The variations in activities of serum immunity factors including superoxide dismutase(SOD),acid phosphatase(ACP),alkaline phosphatase(AKP),peroxide catalase(CAT) were assayed.Statistical analysis showed that SOD activity in the infected C.japonica increased in 12 h,24 h,48 h after injection,higher than that in the control group,and reached maximum in 48 h.The CAT activity increased significantly and reached maximum in 12 h.The AKP activity increased in 5 h,12 h,24 h,higher than that in the control group,and reached maximum in 24 h.The ACP activity begun to rise in 12 h,and reached highest activity in 24 h.The results indicated that in 48 h of artificial V.anguillarum infection,sero-immunity factors in C.japonica varied significantly.The activities of SOD,ACP,AKP,and CAT in blood serum increased significantly in different time periods and were higher than that in the control group.