鲮鱼肝细胞超微结构研究

应用透射电镜对鲮鱼肝脏细胞进行了观察。结果表明, 正常状态下肝细胞具有典型的单核现象, 核仁、核膜、核孔等都清晰可见, 肝细胞含有线粒体、粗面内质网、溶酶体、糖原等细胞器和内容物, 胆小管主要为胞内胆小管。饥饿4周后, 鲮鱼肝细胞所含的细胞器总数目减少, 核周粗面内质网含量降低, 线粒体出现多种形状, 其间电子密度较低, 嵴更加稀疏, 溶解, 无全嵴, 其最大变化是肝细胞内的糖原几乎消失, 出现大量的溶酶体。再恢复投喂4周后, 鲮鱼肝细胞各种细胞器均恢复到正常水平, 鲮鱼的糖原大量出现, 占据细胞大量空间。表明鲮鱼肝细胞代谢与细胞器数目和内容物密切相关, 在一段时间的饥饿胁迫之后, 其肝脏能过量地贮存糖原作为能源物质。

The Hepatic UItrastructure of the Mud Carp ( Cirrhinus chinensis Gvnther)

The hepatic ultrastructure in mullet have investigated using transmission electron microscopy . The result shows that the hepatocyte in mullet contain a nucleus . Pronounced nucleoli, Nuclear membrane and Nuclear pore were observed in each nucleus. Mitochondria , rough endoplasmic reticulum , lysosome and glycogen were abundant in cytoplasm of hepatocytes. The intracellular bile cannliculi were observed in mullet. After starvation for 4 weeks , the total number of cell organelles , the number of Rough Endoplasmic Reticulum and mitochondria decreased and the the latter appeared atrophy and had all kinds of forms. The most significant difference compared with the controls was that the number of lysosome increased dramatically and almost all glycogen disappeared. After refeeding for 4 weeks, the quality of cell organelle, and glycogen rose again , however, the number of lysosome fell significantly . These phenomena demonstrated that the quantities and the forms of cell organelles are closely related to the metabolism of hepatocyte.