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IHB系稀有鮈鲫遗传结构研究
引用本文:李慧慧,王春伶,王剑伟.IHB系稀有鮈鲫遗传结构研究[J].水生态学杂志,2018,39(1):83-90.
作者姓名:李慧慧  王春伶  王剑伟
作者单位:中国科学院水生生物研究所,中国科学院水生生物研究所,中国科学院大学;中国科学院水生生物研究所
基金项目:国家科技支撑计划(2011BAI15B01-41);湖北省科技支撑计划2015BCE098。
摘    要:分析IHB系稀有鮈鲫在繁育过程中的遗传结构变化,旨在鉴定IHB系的遗传质量,为实验动物封闭群的建群和维持以及濒危物种种群保护提供参考和借鉴。IHB系稀有鮈鲫建群原代(P0)为2006年采自四川省汉源县的野生个体,采用最佳避免近交法繁育出F1~F8世代,利用12对微卫星位点对遗传结构进行分析。在IHB系培育过程中,平均观测等位基因数从4.4个降低为4.1个,平均有效等位基因数从4.0个降低为3.0个,平均期望杂合度从0.5878降低到0.5518,平均多态信息含量从0.5509降到0.5293;随着传代的持续,后代与建群原代之间的遗传距离与分化程度有逐渐增大趋势,但相邻世代间的差异逐渐减小;繁育过程中共丢失了6个等位基因,从F5代后所有等位基因频率均无显著变化。经过8代的繁育,IHB系遗传多样性虽略有下降,但仍维持适宜的水平,近交程度得到有效控制,且遗传结构趋于稳定,符合实验动物封闭群遗传质量要求。IHB系可作为稀有鮈鲫地模种群的活体基因库。

关 键 词:IHB系稀有鮈鲫  实验动物封闭群  遗传质量控制  微卫星DNA标记  遗传结构
收稿时间:2016/6/3 0:00:00
修稿时间:2018/1/26 0:00:00

Genetic Structure of the IHB Rare Minnow (Gobiocypris rarus)
LI Hui-hui,WANG Chun-lin and WANG Jian-wei.Genetic Structure of the IHB Rare Minnow (Gobiocypris rarus)[J].Journal of Hydroecology,2018,39(1):83-90.
Authors:LI Hui-hui  WANG Chun-lin and WANG Jian-wei
Institution:Institute of Hydrobiology,Chinese Academy of Sciences,Institute of Hydrobiology, Chinese Acedemy of Sciences,University of Chinese Academy of Sciences;Institute of Hydrobiology,Chinese Academy of Sciences
Abstract:In the present study,12 microsatellite markers were used to reveal genetic structure of IHB rare minnow (Gobiocypris rarus), a closed colony of laboratory animal. During the breeding from the founder of the colony to the 8th generation, the number of alleles per locus and average effective number of alleles per locus decreased from 4.4 to 4.1 and 4.0 to 3.0, respectively. Meanwhile, average expected heterozygosity and the polymorphic information also decreased, separately from 0.5878 to 0.5518 and 0.5509 to 0.5293. Accompany with the breeding process from generation to generation, genetic distance and genetic variation (Fst) tended to increase between the founder and the last generation, but decrease between the two successive generations. A total of 6 allelic genes were lost from the IHB colony. No significant changes were found on gene frequencies after the 5th generations. Although the genetic diversity declined slightly, a relatively high and acceptable level was maintained. Inbreeding was controlled effectively, and therefore genetic structure of IHB colony tended to be stable. The results showed that the genetic quality of IHB colony meet the requirement of laboratory animal. The IHB colony not only could be used as laboratory animal, but also an in vivo gene pool for the topotype population of G. rarus. The authors discussed the protection inspiration for other endangered aquatic animal.
Keywords:IHB rare minnow  closed colony of laboratory animal  genetic quality control  microsatellite marker  genetic structure
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