| 镉胁迫诱导胭脂鱼血细胞凋亡的研究 |
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| 引用本文： | 陈昌玉,周传江,浦德永.镉胁迫诱导胭脂鱼血细胞凋亡的研究[J].水生态学杂志,2009,30(4):98-102. |
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| 作者姓名： | 陈昌玉 周传江 浦德永 |
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| 作者单位： | 西南大学生命科学学院,三峡库区生态环境教育部重点实验室水产科学重庆市市级重点实验室,重庆,400715 |
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| 基金项目： | 三峡库区生态环境教育部重点实验室开放基金 |
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| 摘 要： | 以胭脂鱼(Myxocyprinus asiaticus)离体血细胞为实验材料,用不同浓度的氯化镉溶液处理.通过瑞士姬姆萨和AO/EB荧光染色,在光学和荧光显微镜下观察了血细胞的形态变化,并统计了细胞的凋亡率.结果显示,在氯化镉的胁迫作用下,胭脂鱼血细胞呈现细胞变圆、核变圆、核边缘化、形成凋亡小体等细胞凋亡的形态特征.较低Cd2+浓度(0.01 mmoL/L)胁迫,1 h即可产生明显的细胞凋亡诱导效应,并且细胞凋亡率随着Cd2+浓度的增加和作用时间的延长而升高.表明Cd2+能诱导胭脂鱼血细胞发生典型的凋亡,而且细胞凋亡率与Cd2+浓度和处理时间成明显的剂量-效应关系.
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| 关 键 词： | 血细胞 镉 细胞凋亡 胭脂鱼 |
| 收稿时间： | 2008/11/10 0:00:00 |
Apoptosis Induction of Blood Cells in Myxocyprinus asiaticus by Cadmium Stress |
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| CHEN Chang-yu,ZHOU Chuan-jiang and PU De-yong.Apoptosis Induction of Blood Cells in Myxocyprinus asiaticus by Cadmium Stress[J].Journal of Hydroecology,2009,30(4):98-102. |
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| Authors: | CHEN Chang-yu ZHOU Chuan-jiang and PU De-yong |
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| Institution: | School of Life Science , Southwest University , Key Laboratory of Eco-Environments in Three Gorges Reservoir Region(Ministry of Education ), Key Laboratory of Aquatic Science of Chongqing, Chongqing 400715 , China,School of Life Science , Southwest University , Key Laboratory of Eco-Environments in Three Gorges Reservoir Region(Ministry of Education ), Key Laboratory of Aquatic Science of Chongqing, Chongqing 400715 , China and School of Life Science , Southwest University , Key Laboratory of Eco-Environments in Three Gorges Reservoir Region(Ministry of Education ), Key Laboratory of Aquatic Science of Chongqing, Chongqing 400715 , China |
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| Abstract: | |
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| Keywords: | Blood cells Cadmium stress Apoptosis Myxocyprinus asiaticus |
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