乌江网箱养殖患病花鲈的细菌分离与分子鉴定

摘 要：利用微生物学常用的细菌分离、纯化方法从病害花鲈分离出9株菌株，编号分别为Ⅰ、Ⅱ、Ⅲ、Ⅳ、Ⅴ、Ⅵ、Ⅶ、Ⅷ、Ⅸ。对9株菌株的16SrRNA基因序列进行PCR扩增，均得到约600bp的片段。将扩增结果进行测序，测序结果输入到NCBI （http://www.ncbi.nlm.nih.gov/）中，用BLAST工具对序列进行同源性比对分析。结果表明：Ⅰ为杀鲑气单胞菌,同源性98%；II为不动菌属，同源性97%；III为肺炎克雷伯氏杆菌，同源性98%；IV为嗜水气单胞菌，同源性99%；V为希瓦氏菌，同源性99%；VI为腐生葡萄球菌同源性99%；VII为枯草芽孢杆菌，同源性99%；Ⅷ为沙雷氏菌属，同源性95%；Ⅸ为杀鲑气单胞菌，同源性97%。

Isolation and Molecular Identification of Endophytic Bacteria from Lateolabrax japonicus Net Cage Culture in the Wujiang River

Abstract:The present experiment was undertaken with the tranditional microbiology method to isolate the endophytic bacteria from Lateolabrax japonicus of net cage culture in wujiang river and 9 strains were isolated.The 600bp fragments of 16S rRNA gene of nine strains were amplificated by PCR methold. These 600bp sequences were successfully sequenced and were entered into the NCBI (http://www.ncbi.nlm.nih.gov/).Objective to analyse the homology by BLAST tool. It shows that nine stains are Aeromonas salmonicida (homology of 98%), Acinetobacter (homology of 97%), Klebsiella pneumoniae (homology of 98%), Aeromonas hydrophila (homology of 99%), Shewanella baltica (homology of 99%), Staphylococcus saprophyticus (homology of 99%), Bacillus subtilis (homology of 99%), Serratia proteamaculans(homology of 95%) and Aeromonas salmonicida (homology of 97%) respectively.