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圆口铜鱼维氏气单孢菌的分离鉴定及药敏试验
引用本文:曹恒源,乔晔,陈小娟,潘磊,谢建军,董纯,安苗,王小明,杨少荣.圆口铜鱼维氏气单孢菌的分离鉴定及药敏试验[J].水生态学杂志,2016,37(4):95-100.
作者姓名:曹恒源  乔晔  陈小娟  潘磊  谢建军  董纯  安苗  王小明  杨少荣
作者单位:贵州大学动物科学学院,贵阳 550025;水利部中国科学院水工程生态研究所, 水利部水工程生态效应与生态修复重点试验室, 武汉 430079,水利部中国科学院水工程生态研究所, 水利部水工程生态效应与生态修复重点试验室, 武汉 430079,水利部中国科学院水工程生态研究所, 水利部水工程生态效应与生态修复重点试验室, 武汉 430079,水利部中国科学院水工程生态研究所, 水利部水工程生态效应与生态修复重点试验室, 武汉 430079,水利部中国科学院水工程生态研究所, 水利部水工程生态效应与生态修复重点试验室, 武汉 430079,水利部中国科学院水工程生态研究所, 水利部水工程生态效应与生态修复重点试验室, 武汉 430079,贵州大学动物科学学院,贵阳 550025;,贵州大学动物科学学院,贵阳 550025;,中国三峡建设管理有限公司, 成都 610041
基金项目:圆口铜鱼人工繁殖技术研究(2) ( JGJ /0242013)
摘    要:从濒死圆口铜鱼的脾和肾病灶部位分离优势菌,进行纯化分离并开展生理生化鉴定、16S r DNA序列分析以及常用抗生素药物的药敏性筛选,以期丰富圆口铜鱼病害防治的基础资料。分离出的1株优势菌在BHI固体培养基上长出表面光滑的灰白色圆形菌落,边缘整齐、不透明、稍隆起;序列长度为1 399 bp,与维氏气单孢菌的同源性为100%,与维氏气单孢菌(KT964297.1;KJ650080.1;KM362731.1)聚为一支,置信度99。结合菌落形态特征和生理生化特征,鉴定菌株为维氏气单孢菌。该菌株对氟苯尼考等7种药物敏感,对利福平中度敏感,对新霉素等9种药物耐药。

关 键 词:圆口铜鱼  维氏气单胞菌  生化鉴定  16S  rDNA序列  药敏试验
收稿时间:5/6/2016 12:00:00 AM
修稿时间:2016/7/28 0:00:00

Isolation, Identification and Drug Susceptibility Test of Aeromonas veronii from Coreius guichenoti
CAO Heng-yuan,QIAO Ye,CHEN Xiao-juan,PAN Lei,XIE Jian-jun,DONG Chun,AN Miao,WANG Xiao-ming and YANG Shao-rong.Isolation, Identification and Drug Susceptibility Test of Aeromonas veronii from Coreius guichenoti[J].Journal of Hydroecology,2016,37(4):95-100.
Authors:CAO Heng-yuan  QIAO Ye  CHEN Xiao-juan  PAN Lei  XIE Jian-jun  DONG Chun  AN Miao  WANG Xiao-ming and YANG Shao-rong
Institution:College of Animal Science, Guizhou University, Guiyang 550025, China;Key Laboratory of Ecological Impacts of Hydraulic-projects and Restoration of Aquatic Ecosystem of Ministry of Water Resources, Institute of Hydroecology, Ministry of Water Resources and Chinese Academy of Sciences, Wuhan 430079, China,Key Laboratory of Ecological Impacts of Hydraulic-projects and Restoration of Aquatic Ecosystem of Ministry of Water Resources, Institute of Hydroecology, Ministry of Water Resources and Chinese Academy of Sciences, Wuhan 430079, China,Key Laboratory of Ecological Impacts of Hydraulic-projects and Restoration of Aquatic Ecosystem of Ministry of Water Resources, Institute of Hydroecology, Ministry of Water Resources and Chinese Academy of Sciences, Wuhan 430079, China,Key Laboratory of Ecological Impacts of Hydraulic-projects and Restoration of Aquatic Ecosystem of Ministry of Water Resources, Institute of Hydroecology, Ministry of Water Resources and Chinese Academy of Sciences, Wuhan 430079, China,Key Laboratory of Ecological Impacts of Hydraulic-projects and Restoration of Aquatic Ecosystem of Ministry of Water Resources, Institute of Hydroecology, Ministry of Water Resources and Chinese Academy of Sciences, Wuhan 430079, China,Key Laboratory of Ecological Impacts of Hydraulic-projects and Restoration of Aquatic Ecosystem of Ministry of Water Resources, Institute of Hydroecology, Ministry of Water Resources and Chinese Academy of Sciences, Wuhan 430079, China,College of Animal Science, Guizhou University, Guiyang 550025, China;,College of Animal Science, Guizhou University, Guiyang 550025, China; and China Three Gorges Development Co., Ltd. Chengdu 610041, P.R. China
Abstract:The early life resource of Coreius guichenoti is decreasing significantly year by year in its primary habitat, the middle Jiangsha River, and this is arousing concern about the need for conservation. Fish disease is the primary problem for domestication and propagation of Coreius guichenoti. In July, 2015, an unknown bacterial disease broke out in the domesticated Coreius guichenoti in Wuhan, resulting in the death of over ten fish in a short time. To identify the pathogen and explore prevention strategies, a dominant strain, labeled YT01, was isolated from the spleen and kidney of moribund Coreius guichenoti. Biochemical analysis, 16S rDNA sequencing and an antibiotic susceptibility test were carried out and the data generated will support efforts to control and prevent disease in Coreius guichenoti. The isolated YT01 strain, grown on BHI solid media developed round gray-white colonies with smooth surfaces, neat, opaque and slightly raised. The 16Sr DNA sequence of YT01 strain was 1399 bp long and 100% homologous to Aeromonas veronii. A phylogenic tree was constructed based on the 16S rDNA sequence of the YT01 strain and 16S rDNA sequences from another 25 Aeromonas strains obtained from the NCBI GenBank database. The sequence of the YT01 strain clustered into a clade with Aeromonas veronii (KT964297.1; KJ650080.1; KM362731.1), with a confidence level of 99%. Combined with the morphological characteristics and physiological and biochemical characteristics of the colony, the YT01 strain was conclusively identified as Aeromonas veronii. Antibiotic susceptibility of the YT01 strain was tested using 17 common antibiotics. Results show that the YT01 strain is highly sensitive to 7 antibiotics, including florfenicol, moderately sensitive to rifampin and strongly resistant to 9 antibiotics, including neomycin.
Keywords:Coreius guichenoti  Aeromonas veronii  biochemical identification  16S rDNA sequence  susceptibility test
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