| 草鱼HSP90基因cDNA序列克隆及其组织表达差异 |
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| 引用本文： | 林亚秋,郑玉才,吉红,贺庆华,黄吉芹.草鱼HSP90基因cDNA序列克隆及其组织表达差异[J].水产科学,2009,28(8). |
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| 作者姓名： | 林亚秋 郑玉才 吉红 贺庆华 黄吉芹 |
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| 作者单位： | 1. 西南民族大学,生命科学与技术学院,四川,成都,610041
2. 西北农林科技大学动物科技学院,陕西,杨凌,712100 |
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| 基金项目： | 国家自然科学基金资助项目 |
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| 摘 要： | 根据斑马鱼HSP90(Heat shock protein)序列(NM_131328)设计并合成一对引物,提取草鱼肝脏组织总RNA,RT-PCR扩增获得草鱼HSP90基因cDNA部分序列596 bp,获得GenBank登陆号为FJ517554.将测序结果利用DNAman软件与其他几种已知序列的鱼进行比对,结果表明,草鱼HSP90与斑马鱼、鲤、鲋的同源性依次为90%、89%、92%.同时利用半定量(Semi-quantitative,SQ)RT-PCR技术检测HSP90在草鱼脂肪、肌肉、肠、脑、粘液、性腺、鳔、肝脏、心脏、脾脏、鳃、鳍12个组织的表达差异.结果表明,HSP90在草鱼除了鳍外的其他11个组织中均检测到表达,其中在心脏中表达最高,但与鳃、性腺、脑、脾脏中的表达无显著差异(P>0.05),在脂肪、粘液、鳔组织中的表达显著低于其他几种组织(P<0.05),在肝脏、肌肉与肠中的表达无显著差异(P>0.05).
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| 关 键 词： | 克隆 组织差异 草鱼 |
Cloning and Tissue Expression of HSP90 Partial cDNA Sequence in Grass Carp |
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| LIN Ya-qiu,ZHENG Yu-cai,JI Hong,HE Qing-hua,HUANG Ji-qin.Cloning and Tissue Expression of HSP90 Partial cDNA Sequence in Grass Carp[J].Fisheries Science,2009,28(8). |
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| Authors: | LIN Ya-qiu ZHENG Yu-cai JI Hong HE Qing-hua HUANG Ji-qin |
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| Institution: | 1.College of Life Science and Technology;Southwest University for Nationalities;Chengdu 610041;China;2.Fishery Sciences Department;College of Animal Science and Technology;Northwest Agriculture & Forestry University;Yangling 712100;China |
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| Abstract: | A pair of primers was designed according to zebra HSP90(Heat shock protein)sequence(NM_131328)and total RNA was extracted from hepatopancrease of grass carp(Ctenopharyngodon idellus).596 bp of grass carp HSP90 gene with GenBank number of FJ517554.was obtained by RT-PCR method It was found that the HSP90 gene of grass carp had 90 %,89 % and 92 % homolog with zebra(C.auratus) using DNAman software.The semi-quantitative(SQ RT-PCR) method was used to detect the mRNA differential expression of HSP 90 in the tiss... |
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| Keywords: | HSP90 |
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