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3种消毒剂对水生动物3种主要致病菌的载体定量杀菌试验
引用本文:徐洋,郝贵杰,沈锦玉.3种消毒剂对水生动物3种主要致病菌的载体定量杀菌试验[J].水产科学,2010,29(2).
作者姓名:徐洋  郝贵杰  沈锦玉
作者单位:浙江省淡水水产研究所,浙江,湖州,313001
摘    要:通过载体定量试验对四羟甲基硫酸磷(THPS)、溴硝醇(BNP)和甲醛溶液的杀菌效果进行测定。结果表明,THPS对于嗜水气单胞菌BSK-10菌株的平均杀灭率在浓度为0.500μl/L时,在THPS与消毒剂接触5 min内即100%被杀灭。而甲醛溶液浓度为0.500μl/L时同样接触5 min杀灭率未达100%;THPS对于爱德华氏菌Et菌株的平均杀灭率与菌株BSK-10相近;而对于哈维氏弧菌GYC1108-1株的平均杀灭率在浓度为1.00μl/L时,在菌与消毒剂接触5 min内即100%被杀灭。而甲醛溶液浓度为0.500μl/L时同样接触5 min杀灭率未达100%。BNP对于菌株BSK-10的平均杀灭率在浓度为16 mg/L时,在菌与消毒剂接触5 min内即100%被杀灭;而对于菌株GYC1108-1的平均杀灭率在浓度为8 mg/L时,在菌与消毒剂接触5 min内即100%被杀灭;BNP对于菌株Et的平均杀灭率与菌株GYC1108-1相近。试验表明THPS相对BNP和甲醛溶液的杀菌效果好,鉴于甲醛溶液本身对水体和水生动物的有害影响,可采用四羟甲基硫酸磷取代甲醛溶液。

关 键 词:四羟甲基硫酸磷  溴硝醇  甲醛溶液  载体定量试验  

Inhibitory Effect of Three Disinfectors on Two Pathogenic Bacteria in Aquatic Animals in a Carrier Quantitative Germicidal Test
XU Yang,HAO Gui-jie,SHENG Jin-yu.Inhibitory Effect of Three Disinfectors on Two Pathogenic Bacteria in Aquatic Animals in a Carrier Quantitative Germicidal Test[J].Fisheries Science,2010,29(2).
Authors:XU Yang  HAO Gui-jie  SHENG Jin-yu
Abstract:The bactericidal performance of Tetrakis Hydroxymethyl Phosphonium Sulfate (THPS),2-Bromo-2-nitro-1.3-Propanediol (BNP) and formalin was studied in a carrier quantitative germicidal test. The results showed that the average inhibition effect of THPS on Aeromonas hydrophila strain BSK-10 was 100% at the concentration of 0.500 μl/L. The average inhibition effect of THPS on the strain GYC1108-1 was 100% at the concentration of 500 μl/L. The average inhibition effect of THPS on Edwarelsiella (Et) was found to be 100% at the concentration of 1.00 μl/L. The average rate of inhibition effect of BNP on BSK-10 was 100% at the concentration of 16 mg/L. The average inhibition effect of BNP on GYC1108-1 was 100% at the concentration of 8 mg/L. The average rate of inhibition effect of BNP on the strain Et was 100% at the concentration of 8 mg/L. The results showed that the germicidal efficacy of THPS was stronger than that of formalin and BNP in the same concentration.
Keywords:THPS  BNP  formalin  carrier quantitative germicidal test  
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