| 超声波平板法检测溶藻弧菌HY9901生物被膜的研究 |
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| 引用本文： | 王蓓,吴灶和,简纪常,鲁义善,汤菊芬,黄郁葱.超声波平板法检测溶藻弧菌HY9901生物被膜的研究[J].水产科学,2009,28(7). |
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| 作者姓名： | 王蓓 吴灶和 简纪常 鲁义善 汤菊芬 黄郁葱 |
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| 作者单位： | 广东海洋大学,水产学院,广东省水产经济动物病原生物学及流行病学重点试验室暨广东省高等学校水产经济动物病害控制重点实验室,广东,湛江,524025 |
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| 基金项目： | 广东省科学技术厅社会发展项目 |
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| 摘 要： | 以溶藻弧菌为研究对象,对定量检测生物被膜的超声波平板法进行条件优化,对优化的超声波平板法与平板擦拭法进行比较,并采用银染法进行验证.试验结果表明,优化的超声波平板法的最佳条件为:超声波功率120 W,处理14 min,处理温度25 ℃,优于平板擦拭法.
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| 关 键 词： | 生物被膜 溶藻弧菌 超声波平板法 优化条件 |
Optimization of Ultrasonic-plate Method for Quantitative Detection of Bacterium Vibrio alginolyticus(HY9901) Biofilm |
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| WANG Bei,WU Zao-he,JIAN Ji-chang,LU Yi-shan,TANG Ju-fen,HUANG Yu-cong.Optimization of Ultrasonic-plate Method for Quantitative Detection of Bacterium Vibrio alginolyticus(HY9901) Biofilm[J].Fisheries Science,2009,28(7). |
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| Authors: | WANG Bei WU Zao-he JIAN Ji-chang LU Yi-shan TANG Ju-fen HUANG Yu-cong |
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| Institution: | WANG Bei,WU Zao-he,JIAN Ji-chang,LU Yi-shan,TANG Ju-fen,HUANG Yu-cong(Fisheries College,Guangdong Ocean University,Guangdong Provincial Key Laboratory of Pathogenic Biology , Epidemiology for Aquatic Economic Animals & Key Laboratory of Disease Controll for Aquatic Economic Animals,Guangdong Higher Education Institutions,Zhanjiang 524025,China) |
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| Abstract: | Biofilms are characterized by widespread and hard to be detected and eliminated,which are potential risk to aquaculture production and fishery product safety.The ultrasonic-plate method was optimized to eliminate bacterium Vibrio alginolyticus as a biofilms model strain and compared with the plate-swabbing method and then the silver-staining method was used for verification.The results showed that the optimal conditions for ultrasonic-plate method were found to be: ultrasonic power of 120 W,treatment time 1... |
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| Keywords: | biofilm Vibrio alginolyticus ultrasonic-plate method optimization |
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