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47种中草药体外抑杀嗜水气单胞菌的药效研究
引用本文:陈霞,张其中,李春涛.47种中草药体外抑杀嗜水气单胞菌的药效研究[J].水产科学,2012,31(7):387-391.
作者姓名:陈霞  张其中  李春涛
作者单位:1. 水产科学重庆市市级重点实验室,三峡库区生态环境教育部重点实验室,西南大学生命科学学院,重庆400715
2. 水产科学重庆市市级重点实验室,三峡库区生态环境教育部重点实验室,西南大学生命科学学院,重庆400715;暨南大学水生生物研究所,广东广州 510632
基金项目:国家"863"计划项目,重庆市科技攻关计划项目,广东省海洋与渔业局科技推广项目,广州市科技支撑计划项目
摘    要:用琼脂扩散法(打孔法)测定了石榴皮等47种中草药对嗜水气单胞菌的抑制作用,并用二倍稀释法测定28种有抑菌作用的中草药对嗜水气单胞菌的最小抑菌质量浓度和最小杀菌质量浓度。结果显示石榴皮的抑菌和杀菌效果最好,其抑菌圈直径超过20mm,最小抑菌质量浓度和最小杀菌质量浓度均为7.81mg/mL;赤芍次之。虎杖等26种中草药有一定的抑菌和杀菌效果;陈皮等19种中草药无抑菌作用。石榴皮对嗜水气单胞菌的体外抑制效果最好。

关 键 词:嗜水气单胞菌  中草药  抑菌效果

Antibacterial Effect of 47 Chinese Herbal Medicines on Bacterium Aeromonas hydrophila in vitro
CHEN Xia , ZHANG Qi-zhong , LI Chun-tao.Antibacterial Effect of 47 Chinese Herbal Medicines on Bacterium Aeromonas hydrophila in vitro[J].Fisheries Science,2012,31(7):387-391.
Authors:CHEN Xia  ZHANG Qi-zhong  LI Chun-tao
Institution:1( 1.Key Laboratory of Fisheries Sciences in Chongqing,Key Laboratory Eco-environments in Three Gorges Reservoir Region,Ministry of Education,School of Life Sciences,Southwest University,Chongqing 400715,China;2.Hydrobiology Institute,Jinan University,Guangzhou 510632,China)
Abstract:The bacteriostatic effects of 47 Chinese herbal medicines including Punica granatum on bacterium Aeromonas hydrophila were detected through diffusion method(punch method),and the minimal inhibitory concentration(MIC) and the minimum bactericidal concentration(MBC) of 28 Chinese herbs were determined by double dilution method.The results showed that most Chinese herbal medicine extracts,especially P.granatum,had the inhibitory effect on A.hydrophila.The P.granatum showed the maximal inhibitory circle of over 20 mm,and both MIC and MBC of 7.81 mg/mL,followed by Paeonia lactiflora.Except the two species mentioned above,other 26 Chinese herbal medicines,such as Polygonum cuspidatum,had inhibitory effects;and the rest 19 Chinese herbal medicines including Citrus reticulata were found to have resistant to A.hydrophila.Therefore the most effective Chinese herbs against A.hydrophila was P.granatum,which can be applied to control the diseases caused by A.hydrophila.
Keywords:Aeromonas hydrophila  Chinese herbal medicine  antibacterial effect
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