| 驼海燕内脏中蛋白酶的提取及其酶学性质的研究 |
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| 引用本文： | 和树倩,汪秋宽,乔志勇,张琳琳,秦振中,何云海.驼海燕内脏中蛋白酶的提取及其酶学性质的研究[J].水产科学,2012,31(5):298-301. |
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| 作者姓名： | 和树倩 汪秋宽 乔志勇 张琳琳 秦振中 何云海 |
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| 作者单位： | 大连海洋大学 辽宁省水产品加工及综合利用重点实验室,辽宁 大连,116023 |
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| 摘 要： | 以驼海燕为原料,用丙酮沉淀法制备蛋白酶粗酶,并对其酶学性质进行研究。试验结果表明,提取蛋白粗酶的最佳条件为:原酶液与丙酮的体积比为1∶1.6;蛋白酶反应的最适温度为45℃,最适pH值为8.1;pH为7～9时可保持较高的酶活力;蛋白酶的酶活力在20～50℃基本稳定。酶促反应结果表明,Na+、K+、Li+、Mg2+对蛋白酶活力具有促进作用,而Zn2+、Cu2+、Hg2+能抑制蛋白酶的活力,EDTA对蛋白酶活力的影响甚微。酶动力学方程表明,最大反应速度vmax=5.99U/mL,米氏常数Km=0.69g/mL。
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| 关 键 词： | 驼海燕内脏 蛋白酶 酶制备 酶学性质 |
Collection and Characteristics of Protease Distilled from Viscus of Starfish Asterina gibbosa |
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| HE Shu-qian
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WANG Qiu-kuan
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QIAO Zhi-yong
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ZHANG Lin-lin
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QIN Zhen-zhong
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HE Yun-hai.Collection and Characteristics of Protease Distilled from Viscus of Starfish Asterina gibbosa[J].Fisheries Science,2012,31(5):298-301. |
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| Authors: | HE Shu-qian WANG Qiu-kuan QIAO Zhi-yong ZHANG Lin-lin QIN Zhen-zhong HE Yun-hai |
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| Institution: | (The Key and Open Laboratory of Fishery Product Processing and Utilization of Liaoning Province, Dalian Fisheries University,Dalian 116023,China) |
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| Abstract: | The protease was distilled from viscus of starfish Asterina gibbosa by acetone precipitation and the characteristics of the protease were studied.The protease enzyme yields and the activity of the enzyme revealed that the optimal extraction of the protease was carried out at the ratio of the crude enzyme fluid to acetone volume= 1∶1.6,temperature of 45 ℃,and pH of 8.1.The protease enzyme showed high activity at pH of 7~9,and constant activity at temperature of 20~50 ℃.The Na+,K+,Li+,and Mg2+ were shown improvement of the enzyme activity while Zn2+,Cu2+,and Hg2+ had suppression for the enzyme activity.There was slight influence of EDTA on the enzyme activity whose dynamic equation was expressed as v max of 5.99 U /mL,and Km of 0.69 mg/mL. |
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| Keywords: | viscus of Asterina gibbosa protease enzyme extracted enzyme characteristics |
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