| 鲤、鲫杂交子代的同工酶分析 |
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| 引用本文： | 鲍迪,梁爱军,董莹,王淞,金万昆,董仕.鲤、鲫杂交子代的同工酶分析[J].水产科学,2012,31(5):283-287. |
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| 作者姓名： | 鲍迪 梁爱军 董莹 王淞 金万昆 董仕 |
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| 作者单位： | 1. 天津师范大学 生命科学学院,天津市细胞遗传与分子调控重点实验室,天津 300387
2. 国家级天津市换新水产良种场,天津,301500 |
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| 基金项目： | 天津市科技支撑计划项目，天津师范大学校内基金资助项目 |
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| 摘 要： | 利用水平式淀粉凝胶电泳法对乌克兰鳞鲤(♀)×乌龙鲫四倍体(♂)、红鲫(♀)×乌龙鲫四倍体(♂)、红鲫(♀)×乌龙鲫二倍体(♂)、白鲫(♀)×墨龙鲤(♂)4组鲤鱼、鲫鱼杂交子代背侧肌肉组织的天冬氨酸转氨酶、α-甘油醛磷酸脱氢酶、葡萄糖磷酸异构酶、异柠檬酸脱氢酶、乳酸脱氢酶、苹果酸脱氢酶、磷酸葡萄糖变位酶及肌浆蛋白进行电泳分析,并测量了红细胞长径。红细胞测量结果表明,乌克兰鳞鲤(♀)×乌龙鲫四倍体(♂)、红鲫(♀)×乌龙鲫四倍体(♂)、红鲫(♀)×乌龙鲫二倍体(♂)杂交子代为三倍体,白鲫(♀)×墨龙鲤(♂)杂交子代为二倍体。4组杂交子代葡萄糖磷酸异构酶同工酶的基因组成结果显示,父本乌龙鲫四倍体和父本乌龙鲫二倍体均产生二倍体配子,且二倍体配子中1套为鲤鱼染色体组,1套为鲫鱼染色体组。
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| 关 键 词： | 鲤鱼 鲫鱼 杂交子代 乌龙鲫 同工酶 红细胞长径 |
The Isozyme Electrophoresis in Hybrid of Crucian Carp with Common Carp |
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| BAO Di
,
LIANG Ai-jun
,
DONG Ying
,
WANG Song
,
JIN Wan-kun
,
DONG Shi.The Isozyme Electrophoresis in Hybrid of Crucian Carp with Common Carp[J].Fisheries Science,2012,31(5):283-287. |
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| Authors: | BAO Di LIANG Ai-jun DONG Ying WANG Song JIN Wan-kun DONG Shi |
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| Institution: | 1(1.College of Life Sciences,Tianjin Normal University,Tianjin Key Laboratory of Cyto-Genetical and Molecular Regulation,Tianjin 300387,China;2.National Tianjin Huanxin Aquatic Thoroughbred Hatchery, Tianjin 301500,China) |
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| Abstract: | Electrophoresis of isozymes aspartate aminotransferase(AAT),α-glyerophosphate dehydrogenase(α-GPD),glucose-phosphate isomerase(GPI),isocitrate dehydrogenase(IDH),lactate dehydrogenase(LDH),malate dehydrogenase(MDH),phosphoglucomutase(PGM) and myogen(PROT) were carried out,and the isozymes of erythrocyte were measured in four types hybrids progeny,including Cyprinus carpio(♀)×Carassius auratus auratuswulong crucian carp(F2) 4n](♂),C.auratus red var(♀)×C.auratus auratuswulong crucian carp(F2) 4n](♂),C.auratus red var(♀)×C.auratus auratuswulong crucian arp(F2) 2n](♂) and C.auratus auratus(crucian carp white)(♀)×C.carpio var molong(♂).The long diameter of erythrocyte of C.carpio(♀)×C.auratus auratuswulong crucian carp(F2) 4n](♂),Carassius auratus red var(♀)×C.auratus auratuswulong crucian carp(F2) 4n](♂),C.auratus red var(♀)×C.auratus auratuswulong crucian carp(F2) 2n](♂) belonging to triploid type,and C.auratus auratus(crucian carp white)(♀)×C.carpio var molong(♂) belonging to diploid type.Isozyme analyse revealed that both the wulong crucian carp(F2) 4n and wulong crucian carp(F2) 2n produced diploid spermatozoa,one of genome belonged to crucian carp and the other one belonged to common carp. |
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| Keywords: | hybrid progeny of crucian carp and common carp crucian carp isozyme long diameter |
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