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| 应用Dot-ELISA快速检测牙鲆腹水病病原的研究 | |
| 引用本文： | 朱壮春,陈阳,郝东升,姜广建,邢朝斌,吴鹏,白崇洲,李金龙,史相国.应用Dot-ELISA快速检测牙鲆腹水病病原的研究[J].水产科学,2010,29(3). |
| 作者姓名： | 朱壮春 陈阳 郝东升 姜广建 邢朝斌 吴鹏 白崇洲 李金龙 史相国 |
| 作者单位： | 1. 华北煤炭医学院,生物科学系,河北,唐山,063000 2. 华北煤炭医学院,附属医院,河北,唐山,063000 3. 陡河水库管理处,河北,唐山,063000 |
| 基金项目： | 唐山市科技局科技攻关项目(06125401A-3) |
| 摘 要： | 选用硝酸纤维素膜作固相载体,建立检测迟钝爱德华氏菌的斑点酶联免疫吸附试验诊断方法。根据棋盘试验,确定迟钝爱德华氏菌免疫血清最佳工作浓度为1∶800,酶标抗体最佳工作浓度为1∶200,以出现明显清晰斑点者判定为阳性。用该方法检测时,迟钝爱德华氏菌呈阳性,温和气单胞菌、嗜水气单胞菌、河流弧菌、溶藻弧菌、鳗弧菌、腐败希瓦氏菌、产碱普罗威斯登菌、阪崎肠杆菌和大肠杆菌均呈阴性。试验结果表明,Dot-ELISA方法简便、特异、快速、结果直观,便于在基层推广使用。 |
| 关 键 词： | 斑点酶联免疫吸附试验 牙鲆 迟钝爱德华氏菌 快速检测 |
Rapid Detection of Pathogenic Bacteria of Ascites in Japanese Flounder(Paralichthys olivaceus)by Dot-ELISA |
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| ZHU Zhuang-chun,CHEN Yang,HAO Dong-sheng,JIANG Guang-jian,XING Zhao-bin,WU Peng,BAI Chong-zhou,LI Jin-long,SHI Xiang-guo.Rapid Detection of Pathogenic Bacteria of Ascites in Japanese Flounder(Paralichthys olivaceus)by Dot-ELISA[J].Fisheries Science,2010,29(3). | |
| Authors: | ZHU Zhuang-chun CHEN Yang HAO Dong-sheng JIANG Guang-jian XING Zhao-bin WU Peng BAI Chong-zhou LI Jin-long SHI Xiang-guo |
| Institution: | 1.Department of Biological Sciences;North China Coal Medical College;Tangshan 063000;China;2 Affiliated Hospital of North China Coal Medical College;3.Douhe Reservoir Management;China |
| Abstract: | A Dot-Enzyme Linked Immunosorbent Assay(Dot-ELISA)was successfully established for detection of Edwardsiella tarda found in Japanese flounder(Paralichthys olivaceus)by using nitrocellulose membrane as solid carrier. The working concentration of serum samples was 1∶800 dilution and that of the enzyme-labeled goat anti-rabbit IgG was 1∶200 dilution by chessboard assay. The samples with clear and obvious dots were judged as positive reaction. Edwardsiella tarda was positive in the Dot-ELISA, while Aeromonas sobria, A. hydrophila, Vibrio fluvialis, V.alginolyticus , V.anguillarum , Shewanella putrefaciens, Providencia alcalifaciens , Enterobacter sakazakii and Escherichia coli were negative in the assay. The trial indicated that this method was simple, specific, quick, direct, easy extention and application at basic units. |
| Keywords: | Dot-ELISA Japanese flounder(Paralichthys olivaceus) Edwardsiella tarda rapid detection |
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