| 抗生素抑制农杆菌的效果及对条斑紫菜生长发育的影响 |
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| 引用本文： | 王萍,成锋,滕亚娟,李信书,许星鸿.抗生素抑制农杆菌的效果及对条斑紫菜生长发育的影响[J].水产科学,2009,28(7). |
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| 作者姓名： | 王萍 成锋 滕亚娟 李信书 许星鸿 |
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| 作者单位： | 1. 淮海工学院,海洋学院,江苏省海洋生物技术重点建设实验室,江苏,连云港,222005
2. 百奥生物技术(南通)有限公司,江苏,南通,226010 |
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| 基金项目： | 江苏省海洋生物技术重点建设实验室开放项目，淮海工学院引进人才科研启动基金 |
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| 摘 要： | 用头孢霉素和卡那霉素抑制农杆菌(LBA4404和EHA105)生长,同时测定在液体培养条件下条斑紫菜组织块对2种抗生素的敏感性,以期找到适合用于农杆菌介导条斑紫菜遗传转化的脱菌剂及农杆菌转化用于条斑紫菜的可行性.试验结果表明,2种抗生素对2株农杆菌菌株有较强的抑制作用,对条斑紫菜叶状体组织块的生长作用效果不同,头孢霉素≥300 mg/L抑制叶状体组织块长度的增加,而卡那霉素≥400 mg/L对组织块的增长有一定的促进作用.头孢霉素对条斑紫菜的发育抑制作用较卡那霉素更大.在培养液中添加头孢霉素≥300 mg/L或卡那霉素质量浓度≥600 mg/L组织块不能发育形成丝状体.头孢霉素和卡那霉素可作为农杆菌介导条斑紫菜遗传转化的抑菌剂,使用较适宜的质量浓度分别为200 mg/L和400 mg/L.
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| 关 键 词： | 条斑紫菜 农杆菌 抗生素 生长与发育 |
The Effect of Antibiotics Inhibiting Agrobacterium and the Influence on the Growth and Development in Laver Porphyra yezoensis |
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| WANG Ping,CHENG Feng,TENG Ya-juan,LI Xin-shu,XU Xing-hong.The Effect of Antibiotics Inhibiting Agrobacterium and the Influence on the Growth and Development in Laver Porphyra yezoensis[J].Fisheries Science,2009,28(7). |
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| Authors: | WANG Ping CHENG Feng TENG Ya-juan LI Xin-shu XU Xing-hong |
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| Institution: | WANG Ping 1,CHENG Feng 2,TENG Ya-juan 1,LI Xin-shu 1,XU Xing-hong 1 (1.School of Marine Science & Technology,Huaihai Institute of Technology,Jiangsu Key Laboratory of Marine Biotechnology,Lianyungang 222005,China,2.Biomics Biotechnologies(Nantong) Co.Ltd.,Nantong 226010,China) |
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| Abstract: | Cefotaxime and kanamycin sulfate were used to inhibit the growth of Agrobacterium(LBA4404 and EHA105)and the sensitivity of thallus of laver Porphyra yezoensis cultured in the liquid media to two kinds of antibiotics were measured in order to investigate the bacteria-elimination agent which mediated genetic transformation in the laver via Agrobacterium-medium and to verify the feasibility.The results showed that the two kinds of antibiotics had a strong inhibition on the growth of Agrobacteriu m and a diffe... |
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| Keywords: | Porphyra yezoensis Agrobacterium antibiotics growth and development |
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