急性温度胁迫对太平洋鳕仔稚鱼成活率、生理生化指标的影响

为探究温度胁迫对太平洋鳕的影响,以太平洋鳕仔稚鱼为研究对象,研究了温度胁迫对太平洋鳕仔稚鱼的成活率、总超氧化物歧化酶、过氧化氢酶、丙二醛的活性的影响。试验结果表明,培育温度8℃组(对照组)成活率最高(99%),15℃组的成活率最低(43.3±10.3)%,差异显著(P0.05)。2、5、11、15℃胁迫组的总超氧化物歧化酶活性在24h后急剧升高,与8℃组差异显著(P0.05)。72h后各组总超氧化物歧化酶活性开始下降,与8℃组差异不显著(P0.05)。各胁迫组的过氧化氢酶活性在48h时开始显著升高(P0.05),表明过氧化氢酶活性的变化较总超氧化物歧化酶相比具有滞后性。丙二醛活性在胁迫24h时升高(P0.05),但在48h时略有回落,72h时表现出最大的活性。

The Effects of Sharply Changes in Temperature on Survival and Indices of Physiology and Biochemistry in Pacific Cod Gadus macrocephalus

The effects of temperature stress on survival and activities of total superoxide dismutase(SOD),and catalase(CAT) and contents of malonaldehyde(MDA) were studied in larval Pacific cod Gadus macrocephalus.The results showed that the maximal survival rate(99%) was observed in the group 8 ℃ and the minimal(43.3±10.3)% in the group 15 ℃(P<0.05).The sharp increase in SOD activity was found in the groups 2 ℃,5 ℃,11 ℃,and 15 ℃ for 24 h,significant difference from that in the group 8 ℃(P<0.05).The SOD activity was decreased at 72 h without significant difference from that in the group 8 ℃(P>0.05).The CAT activity was shown to be significantly increased at 48 h(P<0.05),indicating that CAT was hysteretic compared with SOD activity.The content of MDA was found to be increased at 24 h(P<0.05),but slightly to be decreased at 48h.The maximal content of MDA was observed at 72 h.