| 虾夷扇贝线粒体非编码区2(NcR2)序列多态性研究 |
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| 引用本文： | 刘莹,刘卫东,高祥刚,赫崇波.虾夷扇贝线粒体非编码区2(NcR2)序列多态性研究[J].水产科学,2009,28(5). |
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| 作者姓名： | 刘莹 刘卫东 高祥刚 赫崇波 |
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| 作者单位： | 1. 辽宁师范大学,生命科学学院,辽宁,大连,116029;辽宁省海洋水产科学研究院,辽宁省海洋水产分子生物学重点实验室,辽宁,大连,116023
2. 辽宁省海洋水产科学研究院,辽宁省海洋水产分子生物学重点实验室,辽宁,大连,116023 |
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| 基金项目： | 海洋公益行业科研专项资助项目，辽宁省科学技术计划重大、重点项目，辽宁省海洋与渔业科研计划项目 |
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| 摘 要： | 利用PCR产物直接测序法对大连獐子岛(ZZD)、大连旅顺口(LSK)、日本青森县(QSX)及俄罗斯海森崴(HSW)4个群体40个虾夷扇贝的线粒体DNA序列变异进行分析.共测定了40个序列,得到14个单元型.除NcR2序列外,其他序列无变异存在.在NcR2序列中,有15个变异位点,包括9个转换位点、2个颠换位点、4个插入/缺失位点.ZZD群体含有的单元型最多,为6种,HSW群体含有5种,LSK群体和QSX群体各含有3种,大连的2个群体中均具有与日本群体相同的单元型H2,表明它们之间关系较近.ZZD群体的单元型多态性比例最高(60%),HSW次之,LSK与QSX群体最低,均为30%,说明獐子岛群体的遗传多样性较高.
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| 关 键 词： | 虾夷扇贝 线粒体DNA NcR2序列 单元型 |
Sequence Analysis of Non-coding Region 2(NcR2) in mtDNA of Japanese Scallop(Patinopecten yessoensis) |
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| LIU Ying,LIU Wei-dong,GAO Xiang-gang,HE Chong-bo.Sequence Analysis of Non-coding Region 2(NcR2) in mtDNA of Japanese Scallop(Patinopecten yessoensis)[J].Fisheries Science,2009,28(5). |
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| Authors: | LIU Ying LIU Wei-dong GAO Xiang-gang HE Chong-bo |
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| Institution: | 1.College of Life Science;Liaoning Normal University;Dalian 116029;China;2.Liaoning Ocean and Fisheries Science Research Institute;Liaoning Key Laboratory of Marine Fishery Molecular Biology;Dalian 116023;China |
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| Abstract: | Analysis of sequence variation in a mitochondrial DNA Non-coding Region 2(NcR2) was conducted in 40 individuals of four populations of Japanese scallop(Patinopecten yessoensis)from Dalian(Zhangzhidao,ZZD and Lvsun,LSK),Japan(Aomori,QSX)and Russia(Vladivostok,HSW).Fifteen variable sites including nine transitions,two transversions and four indels were found from the NcR2 segment of 14 haplotypes,of which six were observed in ZZD,five in HSW,three in LSK and QSX populations.Haplotype H2 was shared by populati... |
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