酶法提取4种淡水鱼鱼鳞胶原蛋白的研究

对4种常见淡水鱼鱼鳞的基本成分和氨基酸组成进行了测定,研究了酶解鱼鳞制备水解胶原蛋白的工艺,并采取正交试验对水解条件进行了优化。试验结果表明,淡水鱼鳞主要由蛋白质和灰分组成,粗蛋白含量高达55%以上;氨基酸组成显示淡水鱼鳞羟脯氨酸含量丰富,是制备水解胶原蛋白的良好原料。正交试验结果表明,55℃、E/S3%、酶解2h、料液比1∶10(即底物质量浓度为100g/L)时水解效果最好,此时,水解产物分子量较小,且分布集中。

Preparation of Scale Collagen from Four Species Freshwater Fish by Enzymatic Hydrolysis

The general composition and amino acid composition were determined,and the enzymatic hydrolysis technology of collagen was optimized in common freshwater fish scales. The scales were found to be mainly composed of protein(more than 55%)and inorganic substances. There was rich in hydroxyproline in the composition of amino acid in the freshwater fish scales. The optimal conditions had been showed by orthogonal tests as the following:the substrate at a rate of 100 g/L,hydrolyzing at temperature of 55 ℃,and ratio of enzyme to substrate equaling to 3.0 %. In this condition,the hydrolytic collagen had small molecular weight and the central distribution.