普安银鲫胚胎发育中脂质代谢酶基因的表达及葡萄糖的影响

采用荧光定量PCR技术检测了普安银鲫胚胎发育中乙酰辅酶A羧化酶α、脂肪酸合成酶、脂蛋白脂酶和肝脂酶基因表达的特点及葡萄糖浸泡的影响,以探讨乙酰辅酶A羧化酶α、脂肪酸合成酶、脂蛋白脂酶和肝脂酶基因在普安银鲫胚胎发育中的表达及mRNA表达水平及葡萄糖对这4种基因mRNA表达量的影响。试验结果显示,乙酰辅酶A羧化酶α、脂肪酸合成酶、脂蛋白脂酶和肝脂酶基因在普安银鲫的成熟卵期均有表达,且随胚胎发育而上升。在15g/L葡萄糖处理组中,乙酰辅酶A羧化酶α、脂肪酸合成酶、脂蛋白脂酶和肝脂酶从原肠期至出膜前期mRNA表达量升高。在原肠期、晶体出现期和出膜前期,葡萄糖能显著上调脂肪酸合成酶和乙酰辅酶A羧化酶αmRNA的表达(P0.05)。在原肠期,葡萄糖能稍上调脂蛋白脂酶和肝脂酶mRNA的表达,但差异不显著(P0.05)。在晶体出现期和出膜前期,葡萄糖能显著上调脂蛋白脂酶和肝脂酶mRNA的表达(P0.05)。研究表明,乙酰辅酶A羧化酶α、脂肪酸合成酶、脂蛋白脂酶和肝脂酶属母源性酶类,其合子基因在原肠期已经开始表达。适宜水平的葡萄糖促进脂肪酸合成酶和乙酰辅酶A羧化酶αmRNA的表达有利于脂肪酸合成酶和乙酰辅酶A羧化酶α的积累,为仔鱼出膜做准备;促进脂蛋白脂酶和肝脂酶mRNA表达有利于脂质的分解供能。

Expression and Effects of Glucose on Lipid Metabolism Genes during Embryonic Development of Crucian Carp Carassius auratus gibelio in Pu′an County in Guizhou Province

Fluorescence quantitative PCR techniques were used to detect the gene expression of acetyl coenzyme A carboxylase α (ACCα), fatty acid synthase (FAS), lipoprotein lipase (LPL) and hepatic lipase (HL) and the effects of glucose solution treatment on the expression of the above genes during embryonic development of crucian carp Carassius auratus gibelio in Puan County in Guizhou Province.The results showed that there were expressions of FAS, ACCα, LPL and HL genes in the mature egg-stage, and that the expressions exhibited an increasing trend during the embryonic development.In the 15 g/L glucose group, the expressions of FAS, ACCα, LPL and HL mRNA all showed up-rugated changes from gastrula stage to the early hatching, and glucose significantly heightened the expression of FAS and ACCα mRNA (P<0.05).In the gastrula stage, glucose slightly improved the expressions of LPL and HL mRNA, without significant difference (P>0.05).However, glucose led to significantly heighten the expressions of LPL and HL mRNA in the lense appearance stage and hatching (P<0.05).The findings indicate that FAS, ACCα, LPL and HL are maternal enzymes, and the zygotic genes of the four kinds of enzymes in the gastrula stage had already begun to express.Appropriate levels of glucose promoted the expressions of FAS and ACCα mRNA, which is beneficial to the accumulation of FAS and ACCα, in preparation for the larval emergence.Promoting expressions of LPL and HL mRNA facilitated lipid breakdown and energy releasing.