| 中国明对虾主要过敏原基因分子克隆与序列分析 |
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| 引用本文： | 周向红,阎斌伦,李士虎.中国明对虾主要过敏原基因分子克隆与序列分析[J].水产科学,2011,30(2):102-106. |
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| 作者姓名： | 周向红 阎斌伦 李士虎 |
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| 作者单位： | 淮海工学院江苏省海洋生物技术重点实验室,江苏连云港,222005 |
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| 基金项目： | "十一五"国家科技支撑计划重大项目，江苏省海洋生物技术重点实验室开放课题 |
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| 摘 要： | 就中国明对虾主要过敏原原肌球蛋白基因进行了分子克隆与序列分析.从中国明对虾肌肉中提取总RNA,反转录合成第一链cDNA;根据原肌球蛋白cDNA的保守序列设计引物并进行PCR扩增,最后测序获得了中国明对虾原肌球蛋白的cDNA序列(GenBank accession:GU233303).该cDNA 序列含有长855nt的完...
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| 关 键 词： | 中国明对虾 过敏原 原肌球蛋白 分子克隆 |
Molecular Cloning and Characterization of the Gene Coding Major Allergen in Chinese Shrimp Fenneropenaeus chinensis |
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| ZHOU Xiang-hong,YAN Bin-lun,LI Shi-hu.Molecular Cloning and Characterization of the Gene Coding Major Allergen in Chinese Shrimp Fenneropenaeus chinensis[J].Fisheries Science,2011,30(2):102-106. |
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| Authors: | ZHOU Xiang-hong YAN Bin-lun LI Shi-hu |
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| Institution: | ZHOU Xiang-hong,YAN Bin-lun,LI Shi-hu (Jiangsu Key Lab of Marine Biotechnology,Huaihai Institute of Technology,Lianyungang 222005,China) |
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| Abstract: | Food allergy is a type I immediate hypersensitivity reactions that are mediated by IgE antibodies,and do harm to human health.Allergic reactions caused by crustaceans such as shrimps and crabs account for a high proportion in those resulted from seafood.Molecular cloning and characterization of tropomyosin gene was studied in Chinese shrimp Fenneropenaeus chinensis.Total RNA was purified from ventral muscle of Chinese shrimp,and reverse transcribed into first strand cDNA.Primers was designed according to co... |
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| Keywords: | Fenneropenaeus chinensis allergen tropomyosin molecular cloning |
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