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| 条斑星鲽和圆斑星鲽线粒体12S rRNA与16S rRNA基因序列及其系统分析 | |
| 引用本文： | 丛林林,宋广军,木云雷,陈姝君,葛陇利,高祥刚,赫崇波.条斑星鲽和圆斑星鲽线粒体12S rRNA与16S rRNA基因序列及其系统分析[J].水产科学,2007,26(7):390-393. |
| 作者姓名： | 丛林林 宋广军 木云雷 陈姝君 葛陇利 高祥刚 赫崇波 |
| 作者单位： | 1. 辽宁师范大学,生命科学学院,辽宁,大连,116029;辽宁省海洋水产科学研究院,辽宁省应用海洋生物技术开放实验室,辽宁省海洋水产分子生物学重点实验室,辽宁,大连,116023 2. 辽宁省海洋水产科学研究院,辽宁省应用海洋生物技术开放实验室,辽宁省海洋水产分子生物学重点实验室,辽宁,大连,116023 3. 辽宁省海洋水产科学研究院,辽宁省应用海洋生物技术开放实验室,辽宁省海洋水产分子生物学重点实验室,辽宁,大连,116023;大连水产学院,生命科学与技术学院,辽宁,大连,116023 |
| 基金项目： | 科技部科技基础条件平台建设计划 |
| 摘 要： | 采用PCR产物直接测序法分别测定了条斑星鲽和圆斑星鲽线粒体基因组序列,并对12SrRNA和16S rRNA基因核苷酸全序列进行分析,条斑星鲽和圆斑星鲽线粒体12S rRNA的核苷酸序列长度分别为948 bp和949 bp,16S rRNA均为1716 bp。试验结果表明,由12S rRNA和16S rRNA基因所构建的两个系统树的结构基本一致,21种鱼主要分为3个大的分支:鲤形目、鲶形目聚为1支,鲑形目独立聚为1支,鲈形目和鲽形目聚为1大支。鲆鲽类与鲈形目的鲹科、鲷科鱼类聚类在一支,且与鲹科的日本竹荚鱼、大西洋竹荚鱼构成姊妹群,支持鲆、鲽类是从鲈形目分化出来的观点,而同属于鲽形目的鳎科鱼类塞内加尔鳎在12S rRNA树中成为一个独立的分支,在16S rRNA树中聚到鲈形目和鲽形目的分支中,并且与鲈形目关系更近些,鳎类是1个特殊类群,其分类地位有待进一步探讨。线粒体基因组序列已提交到GenBank中,序列号为:DQ403797和EF025506。 |
| 关 键 词： | 圆斑星鲽 条斑星鲽 系统发生 |
| 文章编号： | 1003-1111（2007）07-0390-04 |
| 修稿时间： | 2006-10-082006-10-31 |
The 12S and 16S rRNA Sequences and Phylogenetic Analysis of Barfin Flounder Verasper moseri and Spotted Halibut V. variegatus |
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| CONG Lin-lin,SONG Guang-jun,MU Yun-lei,CHEN Shu-jun,GE Long-li,GAO Xiang-gang,HE Chong-bo.The 12S and 16S rRNA Sequences and Phylogenetic Analysis of Barfin Flounder Verasper moseri and Spotted Halibut V. variegatus[J].Fisheries Science,2007,26(7):390-393. | |
| Authors: | CONG Lin-lin SONG Guang-jun MU Yun-lei CHEN Shu-jun GE Long-li GAO Xiang-gang HE Chong-bo |
| Institution: | 1. College of Life Science, Liaoning Normal University, Dalian 116029, China; 2. Liaoning Ocean and Fisheries Science Research Institute, Liaoning Open Lab of Applied Marine Biotechnology, Liaoning Key Lab of Marine Fishery Molecular Biology, Dalian 116023, China; 3. College of Life Science and Technology, Dalian Fisheries University, Dalian 116023, China |
| Abstract: | The 12S and 16S rRNA sequences were amplified in barfin flounder(Verasper moseri) and spotted halibut(V.variegatus) by PCR and directly sequenced.The length of 12S rRNA gene was 948 bp(barfin flounder) and 949 bp(spotted halibut) and 16S rRNA was all 1716 bp in both species.The phylogenetic relationships of fishes in Cypriniformes,Siluriformes,Salmoniformes,Pleuronectiformes and Perciformes were constructed through NJ method using Bos faurus as outgroup.Results showed that Pleuronectiformes and Perciformes formed one large group,with Bothidae,Pleuronectidae and Carangidae forming one group,and Soleidae was merged into the Carangidae and Eleotridae in 16S tree,forming an independent group in 12S tree.GenBank accession No.is DQ403797 and EF025506. |
| Keywords: | 12S rRNA 16S rRNA |
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