| 辽宁沿海海蜇与沙海蜇遗传多样性的AFLP分析 |
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| 引用本文： | 高祥刚,曹洁,董婧,刘星,赫崇波.辽宁沿海海蜇与沙海蜇遗传多样性的AFLP分析[J].水产科学,2009,28(2). |
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| 作者姓名： | 高祥刚 曹洁 董婧 刘星 赫崇波 |
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| 作者单位： | 1. 辽宁省海洋水产科学研究院,辽宁省海洋环境监测总站,辽宁省海洋水产分子生物学重点实验室,辽宁,大连,116023
2. 辽宁省海洋水产科学研究院,辽宁省海洋环境监测总站,辽宁省海洋水产分子生物学重点实验室,辽宁,大连,116023;大连水产学院生命科学与技术学院,辽宁大连,116023 |
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| 基金项目： | 国家海洋908项目辽宁专项基金，辽宁省自然科学基金 |
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| 摘 要： | 海蜇和沙海蛰均为腔肠动物门的大型食用水母,采用AFLP分子标记技术对辽宁沿海的海蜇野生群体、养殖群体和沙海蜇野生群体共90个个体进行了遗传多样性分析.10对引物共得到560个稳定扩增位点.3个群体的多态性位点比例为海蜇野生群体82.05%.海蜇养殖群体78.46%,沙海蜇野生群体74.10%;平均杂合度分别为0.2072,0.1850和0.2116,Shannon多样性指数分别为0.3248、0.2954和0.3262,海蜇野生群体与海蜇养殖群体和沙海蜇野生群体的遗传距离分别为0.0300和0.2702.分析结果表明,3个群体的遗传多样性均保持了相对较高的水平.
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| 关 键 词： | 海蜇 沙海蜇 遗传多样性 AFLP分析 |
AFLP Analysis in Jellyfish Populations of Rhopilema esculentum and Nemopilema nomurai in Liaoning Coast |
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| GAO Xiang-gang,CAO Jie,DONG Jing,LIU Xing,HE Chong-bo.AFLP Analysis in Jellyfish Populations of Rhopilema esculentum and Nemopilema nomurai in Liaoning Coast[J].Fisheries Science,2009,28(2). |
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| Authors: | GAO Xiang-gang CAO Jie DONG Jing LIU Xing HE Chong-bo |
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| Institution: | GAO Xiang-gang1,CAO Jie1,2,DONG Jing1,LIU Xing1,HE Chong-bo1(1.Liaoning Ocean , Fishery Science Research Institute,Liaoning Marine Environmental Monitoring General Station,Liaoning Key Laboratory of Marine Fishery Molecular Biology,Dalian 116023,China,2.College of life Science , Technology of Dalian Fisheries University,China) |
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| Abstract: | Amplified fragment length polymorphism(AFLP) was used to assess the genetic diversity of Jellyfishes Rhopilema esculentum and Nemopilema nomurai in Liaoning Coast.Three populations categorzied were as wild,cultured Rhopilema esculentum and wild Nemopilema nomurai.A total of 560 reproducible bands amplified with ten AFLP primer combinations were obtained from 90(each population containing 30 individuals) individuals.For the three populations,the percent of polymorphic loci(P) was 82.05 %,78.46% and 74.10% rs... |
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| Keywords: | Rhopilema esculentum Nemopilema nomurai genetic diversity AFLP analysis |
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