| 抗菌药联用对海藻希瓦菌的体外抗菌效果 |
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| 引用本文： | 曹海鹏,杨丽君,陈思佳,郭成,杨先乐.抗菌药联用对海藻希瓦菌的体外抗菌效果[J].水产科学,2020,39(2):251-257. |
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| 作者姓名： | 曹海鹏 杨丽君 陈思佳 郭成 杨先乐 |
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| 作者单位： | 上海海洋大学,国家水生动物病原库,上海201306;上海海洋大学,上海水产养殖工程技术研究中心,上海201306;上海海洋大学,水产动物遗传育种中心上海市协同创新中心,上海201306;上海海洋大学,国家水生动物病原库,上海201306;上海海洋大学,水产科学国家级实验教学示范中心,上海201306 |
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| 基金项目： | 国家虾产业技术体系项目(CARS-48);江苏省渔业科技创新与推广专项(Y2018-08);青浦区科技发展基金资助项目(青科发2018-11) |
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| 摘 要： | 为评价硫酸庆大霉素和头孢噻肟钠对水产致病性海藻希瓦菌SFH3的体外联合抗菌效果,以6种渔用抗菌药作为对照,比较测定硫酸庆大霉素、头孢噻肟钠对海藻希瓦菌SFH3的体外抗菌作用,在此基础上进一步采用试管二倍稀释法测定分析硫酸庆大霉素和头孢噻肟钠在不同复配比例下对海藻希瓦菌SFH3的体外联合抑菌作用,并通过时间-杀菌曲线法测定其体外联合杀菌效果。试验结果显示,硫酸庆大霉素和头孢噻肟钠对海藻希瓦菌SFH3的最小抑菌质量浓度为80 mg/L和160 mg/L,抗菌活性明显优于甲砜霉素、多西环素、噁喹酸、磺胺间甲氧嘧啶钠、磺胺甲噁唑、盐酸恩诺沙星等渔用抗菌药。硫酸庆大霉素和头孢噻肟钠在复配比例8∶2和7∶3时,对海藻希瓦菌SFH3的体外联合抑菌效果表现为协同作用,对海藻希瓦菌SFH3的最小抑菌质量浓度达40 mg/L。此外,时间-杀菌曲线表明,40 mg/L硫酸庆大霉素和80 mg/L头孢噻肟钠对海藻希瓦菌SFH3的体外联合杀菌作用表现为协同效果。本研究结果证实,硫酸庆大霉素和头孢噻肟钠联用有利于提升对海藻希瓦菌SFH3的体外抗菌效果,可为研发水产致病性海藻希瓦菌的潜在控制药物提供理论参考。
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| 关 键 词： | 硫酸庆大霉素 头孢噻肟钠 海藻希瓦菌 联合抗菌效果 |
In Vitro Antibacterial Effect of Combination of Antimicrobial Agents against Pathogen Shewanella algae |
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| CAO Haipeng,YANG Lijun,CHEN Sijia,GUO Cheng,YANG Xianle.In Vitro Antibacterial Effect of Combination of Antimicrobial Agents against Pathogen Shewanella algae[J].Fisheries Science,2020,39(2):251-257. |
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| Authors: | CAO Haipeng YANG Lijun CHEN Sijia GUO Cheng YANG Xianle |
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| Institution: | (National Pathogen Collection Center for Aquatic Animals,Shanghai Ocean University,Shanghai 201306,China;Shanghai Engineering Research Center for Aquaculture,Shanghai 201306,China;Shanghai Collaborative Innovation for Aquatic Animal Genetics and Breeding,Shanghai Ocean University,Shanghai 201306,China;National Demonstration Center for Experimental Fisheries Science Education,Shanghai Ocean University,Shanghai 201306,China) |
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| Abstract: | The in vitro antibacterial activity of gentamicin sulfate and cefotaxime sodium against pathogenic Shewanella alage SFH3 isolated from Pacific white leg shrimp Litopenaeus vannamei with black spot disease was assayed in comparison with the six fishery antimicrobials, then the in vitro combined inhibitory effect of gentamicin sulfate and cefotaxime sodium was determined at different combined proportions by the tube double broth dilution method, and the in vitro combined bactericidal effect was further confirmed by time-kill curve assay in order to assess the potential of gentamicin sulfate and cefotaxime sodium in combination against aquatic pathogen S. alage SFH3. It was found that minimum inhibitory concentration(MIC) was 80 mg/L in gentamycin sulfate and 160 mg/L in cefotaxime sodium against the pathogen SFH3, significantly lower than thiamphenicol, doxycycline, oxolinic acid, sodium sulfamonomethoxine, sulfamethoxazole, and enrofloxacin hydrochloride. The combination of gentamicin sulfate and cefotaxime sodium showed synergistic inhibitory activities against S. alage SFH3 at combined proportions of 8∶2, and 7∶3 with MICs of 40 mg/L. In addition, 40 mg/L gentamicin sulfate and 80 mg/L cefotaxime sodium had synergistic bactericidal effect against the pathogen SFH3. This finding confirms that the combination of gentamicin sulfate and cefotaxime sodium can contribute to the in vitro antibacterial activity, which provides scientific reference with the drug control of aquatic pathogen S. alage. |
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| Keywords: | gentamycin sulfate cefotaxime sodium Shewanella algae combined antibacterial effect |
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