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三疣梭子蟹血淋巴细胞及血液生化指标的初步研究
引用本文:陈惠群,金珊,王国良,谢觉晓.三疣梭子蟹血淋巴细胞及血液生化指标的初步研究[J].水产科学,2004,23(6):1-4.
作者姓名:陈惠群  金珊  王国良  谢觉晓
作者单位:宁波大学,生命科学与生物工程学院,浙江,宁波,315211
基金项目:浙江省教育厅科研计划项目(20020384)
摘    要:采用记数板记数,常规法涂片,在光学显微镜下对三疣梭子蟹进行细胞体、细胞核的测量及血淋巴细胞的分类记数,并测定血液生化指标。实验结果表明:雌性梭子蟹血淋巴细胞密度较高,并且血细胞的个体差异较大,小颗粒细胞约占细胞总数的84%,大颗粒细胞约占14%,无颗粒细胞最少,而且变异系数较大;三类细胞的大小无显著差异。三疣梭子蟹血清蛋白含量较低,雌蟹血清中谷丙转氨酶、谷草转氨酶及乳酸氨酶活性远远高于雄蟹。研究结果显示:性腺发育雌蟹的细胞密度及酶活性显著高于雄蟹。

关 键 词:三疣梭子蟹  血淋巴细胞  血液指标
文章编号:1003-1111(2004)06-0001-04
修稿时间:2003年12月15

Hemocytes and Blood Biochemical Values in Portunus trituberculatus
CHEN Hui-qunJIN ShanWANG Guo-liangXIE Jue-xiao.Hemocytes and Blood Biochemical Values in Portunus trituberculatus[J].Fisheries Science,2004,23(6):1-4.
Authors:CHEN Hui-qunJIN ShanWANG Guo-liangXIE Jue-xiao
Abstract:Morphometrical and taxonomical hemocytes were conducted under light microscope using Neubauer's board and smear, and the blood biochemical values were determined in Portunus trituberculatus. The results showed that the female crab had high density of hemocytes in which there was great variation in size among individuals. The small granule hemocytes accounted for up to 84% in total hemocytes, and the large ones comprised about 14%. There was the least agranulocytes in which the significant variation in size was found. There was no significant difference in size among three categories of hemocytes. Portunus trituberculatus had low serum protein and there were significantly higher activities of serum glutamic pyruvic transaminase, glutamic oxalocetic transaminase and lactic dehydrogenase in the female crab than those in the male, indicating that the hemocyte density and the enzymatic activities increase with the gonadal development.
Keywords:Portunus trituberculatus  hemocyte  blood biochemical values
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