3种鳜鱼消化道结构与胃中泌酸胃酶细胞分布比较

利用石蜡切片技术比较了鳜鱼、斑鳜和杂交鳜(斑鳜♀×鳜鱼♂)胃、幽门盲囊、肠道结构特征。3种鳜鱼胃壁均由黏膜层、黏膜下层、肌层组成,黏膜层内能看到固有层;其中,鳜鱼、杂交鳜胃黏膜层相对厚度0.30±0.09、0.30±0.11,斑鳜胃黏膜层相对厚度较薄,约0.21±0.13。肠道由黏膜层、黏膜下层和肌层组成,黏膜层表面含有绒毛,绒毛层含有杯状细胞;鳜鱼与杂交鳜褶皱数、杯状细胞密度间差异不显著(P0.05),但显著大于斑鳜(P0.05)。鳜鱼、斑鳜和杂交鳜幽门盲囊平均数目分别为(2.138±0.173)个/μm2、(0.793±0.132)个/μm2和(1.098±0.218)个/μm2。幽门盲囊结构与肠道相似,3种鳜鱼的褶皱数目差异不显著(P0.05),但斑鳜杯状细胞密度显著小于鳜鱼和杂交鳜(P0.05)。同时,利用原位杂交技术比较了3种鳜鱼胃中泌酸胃酶细胞的分布特征,3个胃蛋白酶原基因探针均出现紫色杂交信号,且均位于胃黏膜层。同种鱼中,不同基因探针杂交信号细胞密度差异不显著(P0.05);不同种类间,相同基因探针杂交信号细胞密度大小顺序均为鳜鱼=杂交鳜斑鳜,且相互间差异显著(P0.05)。消化道结构与泌酸胃酶细胞分布差异为鳜鱼消化生理学比较研究提供了基础资料。

Comparison of Digestive Tract Structure and Distribution of Gastric Oxynticopeptic Cells among Three Mandarin Fish Siniperca Species

Structure characterization of stomach ,intestines and pyloric caecum of Mandarinfish Siniperca chuatsi , S .scherzeri and their hybrid (S .scherzeri ♀ × S .chuatsi ♂) was compared via paraffin section technique .Stom‐ach wall containing mucous ,submucosa ,lamina propria and muscular layers in mandarinfish three species shared the same structure .Relative thickness of mucous layer w as 0 .30 ± 0 .09 in S .chuatsi ,and 0 .30 ± 0 .11 in the hy‐brid ,while relative thickness of S .scherzeri was 0 .21 ± 0 .13 ,much thinner than the other two species .Intestines was found to be consisted of villus ,mucous ,submucosa ,and muscular layers ,and inner wall was comprised of many wrinkles ,and goblet cells in villus layer .There was no significant difference in wrinkle number and density of goblet cells between S .chuatsi and the hybrid(P>0 .05) ,while these data of S .scherzeri were significantly lower than S .chuatsi and the hybrid (P<0 .05) .S .chuatsi ,S .scherzeri and the hybrid had mean pyloric cae‐cum number of 213 .8 ± 17 .3 ,79 .3 ± 13 .2 and 109 .8 ± 21 .8 ,respectively .The structure of pyloric caecum was similar to intestines ,there was no significant difference in wrinkle number among three Siniperca species(P>0 .05) ,The S .scherzeri had significantly lower density of goblet cells than S .chuatsi and the hybrid did(P<0 .05) .The distribution characterization of oxynticopeptic cells was compared among the three Siniperca species via in situ hybridization technique .The deep purple hybridization signals of three pepsinogen probes (PG A1 ,A2 and C) were all detected in the gastric cells of mucous layer .Within the same species ,there was no significant difference among hybridization signal cells of PG A 1 ,A2 and C probes (P>0 .05) .Among three Siniperca spe‐cies ,density of hybridization signal cells by the gene probes (PG A1 ,A2 ,and C) was ranked from high to low as :S .chuatsi=hybrid> S .scherzeri ,without significant difference (P<0 .05) .Structure difference of digestive tract and distribution characterization of oxynticopeptic cells provided some scientific base for further study of fish diges‐tion physiology .