日本蟳消化系统组织结构研究

应用石蜡切片和苏木精—伊红染色技术对日本蟳消化系统组织结构进行研究,以期为日本蟳的人工养殖模式建立和饲料开发提供基础资料。研究结果表明,日本蟳消化系统包括消化道和消化腺两部分。消化道由口、食道、胃、中肠、后肠及肛门组成,消化道管壁由内向外为黏膜层、黏膜下层、肌层和外膜。食道黏膜下层中含有丰富的食道腺,可分泌黏液帮助食物的吞咽;贲门胃黏膜上皮覆盖的几丁质层有刚毛或骨板等构成的胃磨可磨碎食物;幽门胃中几丁质层特化形成密集排列的刚毛,起到过滤的作用;中肠黏膜上皮无几丁质层覆盖,形成纹状缘,可参与食物的吸收。消化腺主要是肝胰腺,肝胰腺为复管状腺,由许多肝小管组成。肝小管黏膜上皮由单层柱状上皮细胞组成,上皮细胞有4种类型:吸收细胞、分泌细胞、纤维细胞和胚细胞。

Histological Observation of Digestive System of Japanese Crab Charybdis j aponica

To establish artificial breeding mode and exploit palatable feeds in production of Japanese crab Charybdis j aponica ,histostructure of its digestive system was studied by paraffin method and H · E dye technology .The digestive system of C .j aponica was composed of digestive tract and digestive gland .The digestive tract was consisted of mouth ,esophagus ,stomach ,midgut ,hindgut and anus ,and histostruc‐ture of digestive tract wall was divided into four layers from inside to outside ,namely the mucosa ,submu‐cosa ,muscular layer and adventitia .Abundant esophageal glands secreting mucus to help animals swallow food were found in esophageal submucosa .The setae or bone plates in chitin covered over epithelial layer of cardiac stomach played a role in grinding food .T he chitin layer in pyloric stomach converted into dense‐ly packed setae by which large food particles were blocked into the midgut .There was no chitin layer cov‐ering over the intestinal epithelium .Striated border of the epithelial cells took participate in the absorption of food .Digestive glands also named as hepatopancreas were complex tubular glands and made up of many small liver tubes .The mucosa epithelium of liver tube was composed of a single layer of columnar cells which were divided into four types :absorptive cell ,secretary cell ,fiber cell and embryonic cell .