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乌龟鲍曼不动杆菌的分离鉴定及药敏试验
引用本文:丁利,黎俊榆,代小梅,汪继超,方振华,史海涛.乌龟鲍曼不动杆菌的分离鉴定及药敏试验[J].水产科学,2016(4):426-430.
作者姓名:丁利  黎俊榆  代小梅  汪继超  方振华  史海涛
作者单位:1. 海南师范大学生命科学学院,海南海口,571158;2. 海南职业技术学院生物工程学院,海南海口,570216
基金项目:国家自然科学基金资助项目(31502036、31372228);海南省自然科学基金资助项目(314076、20153086);海南省高等学校科学研究项目(Hnky2016-15).
摘    要:自患病乌龟肝脏中分离出1株优势菌DL01221,对其进行形态学观察、生化试验、16SrRNA基因序列分析,同时采用Kriby-Bauer纸片扩散法进行药物敏感性试验。试验结果表明,该菌为革兰氏阴性球杆菌,将该菌株16SrRNA序列进行同源性和系统进化树分析,发现它与鲍曼不动杆菌处于同一群,同源性超过98%,结合菌株形态和生化指标,确定该菌株为鲍曼不动杆菌。致病性试验结果显示,其对乌龟具有较强的致病作用;耐药性结果显示其对头孢他啶、卡那霉素及氟罗沙星敏感,对其他多种抗生素耐药。

关 键 词:乌龟  鲍曼不动杆菌  分离鉴定  药敏试验

Isolation,Identification and Drug Sensitivity of Acinetobacter baumannii from Tortoise Mauremys nigricans
Abstract:One predominant bacterial strain named DL01221 was isolated from the liver of diseased tortoise M auremys nigricans ,identified by morphologic observation ,biochemical test ,and sequencing analysis of 16S rRNA ,and then the drug sensitivity was determind by Kirby‐Bauer′s agar diffusion method .Results showed that the strain was gram negative bacterial .By analyzing the homology and phylogenetic relation‐ship of 16S rRNA sequences in the NCBI database ,the homology was found to be more than 98% com‐pared to Acinetobacter baumannii ,which was in the same group with A .baumannii .Combining with the colonial morphology and biochemical results ,the strain was identified as A .baumannii .Inoculating the strain to M . nigricans for lethality indicated that the strain had pathogenicity and the same bacterium could be isolated from these animals .Meanwhile the drug sensitivity test showed the bacterium was sensi‐tive to ceftazidime ,kanamycin and fleroxacin ,and multi‐resistant to most antibiotics .
Keywords:Mauremys nigricans  Acinetobacter baumannii  isolation and identification  drug sensitive test
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