七里海中华绒螯蟹遗传多样性的RAPD和ISSR分析

利用RAPD-PCR及ISSR-PCR两种分子标记技术,分析了七里海中华绒螯蟹第6代繁育群体的遗传多样性。用10个多态性高的RAPD引物对24个七里海中华绒螯蟹样本个体进行扩增,共检测出107个不同的扩增位点,扩增片段大小为300~2300bp。其中多态位点总数为66个,平均多态位点比例为61.32%,群体内香农-维纳多样性值为0.1714。用8个多态性高的ISSR引物对24个个体共检测到106个位点,扩增片段为200~1500bp。其中多态性位点79个,平均多态位点百分率为74.53%,群体内香农-维纳多样性值为0.1778。结果显示,该群体的多态位点比例和香农-维纳多样性值均较大,说明其有较丰富的遗传多样性,同时也说明RAPD和ISSR技术用于七里海中华绒螯蟹核基因组的遗传多样性分析,具有较高的检出率和灵敏度。

Analysis of Genetic Diversity of Chinese Mitten Handed Crab Eriocheir sinensis f rom Qilihai by RAPD and ISSR Markers

Two types of molecular markers ,namely ,Randomly Amplified Polymorphic DNA (RAPD) and Inter‐Simple Sequence Repeat (ISSR) were used to detect the genetic diversities at the intra sixth genera‐tion breeding population of Chinese mitten handed crab Eriocheir sinensis from Qilihai .Using 10 RAPD primers selected from 33 primers ,a total of 107 amplified loci ranging from 300 to 2300 bp were observed from 24 individuals ,and 66 of 107 loci detected were polymorphic with the percent of 61 .32% ,and the Shannon′s index of 18 .3419 .Eight ISSR primers of 50 primers used generated 106 reproducible and stable bands ,ranging from 200 to 1500 bp , 79 of 107 loci detected were polymorphic with the percent of 74 .53% ,and the Shannon′s index of 18 .8425 .These findings indicated that the sixth generation breeding population had high genetic diversity ,and that the proportion of polymorphic loci and Shannon′s index of Qilihai Chinese mitten handed crab were high ,indicating that they have abundant genetic diversity .