脊尾白虾血细胞ESTs的生物信息学与微卫星序列特征分析

对前期测序得到的脊尾白虾血细胞2853条EST序列进行了生物信息学和微卫星序列特征分析。EST序列拼接得到1053条Unigenes,包括329条Contigs和724条Singlets。BLAST分析表明,593(56.3%)条Uingenes与数据库中已知基因具有相似性。KEGG代谢途径分析表明,181条Unigenes映射到120条代谢途径。通过EST-SSR分析,共得到416条微卫星序列,检出率为14.58%。其中,两碱基重复序列374条,占89.90%,AG重复类型最多;三碱基35条,占8.41%,AAT重复类型最多;四碱基7条,占1.68%,AAGT重复类型最多。本研究可为脊尾白虾功能基因资源挖掘及分子标记筛选提供有效数据。

Bioinformatics and Microsatellite Sequences Analysis of EST Sequence in Ridge Tail Shrimp Exopalaemon carinicauda

The analysis of bioinformatics and microsatellite sequences characteristics of expressed sequence tag (EST) sequence were studied ,basing on the hemocyte cDNA library of ridge tail shrimp Exopalaemon carinicauda constructed by our laboratory .The 1053 unique sequences were yielded from 2853 high quality ESTs ,including 329 contigs and 724 singletons .BLAST analysis revealed 593 (56 .3% ) of the unique se‐quences as orthologs of genes from other organisms ,22 (2 .1% ) of which were hypothetical protein ,and the remaining 438 (41 .6% ) had no homology to any sequences .Kyoto Encyclopedia of Genes and Ge‐nomes (KEGG) analysis indicated that 181 of the unique sequences mapped to 120 of metabolic pathways . A total of 416 (14 .58% ) of the microsatellite repeat sequences were found using EST‐SSR analysis .In the 416 repeat sequences ,most was dinucleotide with repeats of 374 (89 .90% ) ,followed by trinucleotide with repeats of 35 (8 .41% ) ,and the third tetranucleotide with repeats of 7 (1 .68% ) .The most common repeat types were AG ,AAT and AAGT repeat .The findings provide important information for screening the functional gene resource and molecular markers in ridge tail shrimp .