黄芩对7种水产动物病原菌的体外抑菌活性研究

采用纸片扩散法和二倍稀释法,利用黄芩水提液对副溶血弧菌、嗜水气单胞菌、鳗弧菌、哈维氏弧菌、温和气单胞菌、金黄色葡萄球菌、大肠杆菌进行最低抑菌质量浓度和最低杀菌浓度的测定。试验结果表明,黄芩对鳗弧菌、嗜水气单胞菌、副溶血弧菌和金黄色葡萄球菌有较强的抑菌效果,最低抑菌质量浓度均为15.63 mg/ml,最低杀菌质量浓度分别为31.25、15.63、31.25、31.25 mg/ml;黄芩对大肠杆菌、温和气单胞菌和哈维氏弧菌的抑菌效果弱于副溶血弧菌、嗜水气单胞菌、鳗弧菌、金黄色葡萄球菌,最低抑菌质量浓度分别为31.25、62.51、31.25 mg/ml,最低杀菌质量浓度分别为62.5、62.5、125 mg/ml。

Antibacterial Activities of Chinese Herb Medicine Scutullaria to 7 Pathogens in Aquatic Animals in Vitro

The minimum inhibitory concentration(MIC) and the minimum bactericidal concentration(MBC) of Chinese herb medicine Scutellaria to seven pathogens in aquatic animals were determined in vitro to develop a new Chinese herbal medicine for aquatic animals by discagar diffusion method.The MIC’s of Vibrio anguillarum,Aeromonas hydrophila,V.parahaemolyticus and Staphylococcus aureus were found to be 15.63 mg/ml,and the MBC 31.25 mg/ml,15.63 mg/ml,31.25 mg/ml and 31.25 mg/ml.Compared with the four pathogens above,Scutellaria was shown to have weaker anterbacterial activities for the rest pathogens,with the MIC of 31.25 mg/ml for Escherichia coli,62.5 mg/ml for A.sobria and 31.25 mg/ml for V.harveyi,and the MBC of 62.5 mg/ml for E.coli,62.5 mg/ml for A.sobria and 125 mg/ml for V.harveyi.