| 不同质量浓度铅对草鱼鱼种抗氧化性能的影响 |
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| 引用本文： | 郑清梅,郭江山,温茹淑,韩春艳.不同质量浓度铅对草鱼鱼种抗氧化性能的影响[J].水产科学,2010,29(11). |
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| 作者姓名： | 郑清梅 郭江山 温茹淑 韩春艳 |
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| 作者单位： | 嘉应学院生命科学学院;梅州市水产研究所; |
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| 基金项目： | 嘉应学院自然科学基金资助项目 |
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| 摘 要： | 将草鱼暴露于不同水平(0、72、144、288 mg/L)的硝酸铅水溶液中,分别于12、24、48、96 h测定草鱼肝、鳃组织中谷胱甘肽过氧化物酶(GSH-Px)、超氧化物歧化酶(SOD)活性和丙二醛(MDA)含量.试验结果表明,当鱼经不同质量浓度Pb2+处理24 h后,其肝脏的GSH-Px活性表现为诱导效应;而鳃在低质量浓度(72 mg/L)Pb2+处理也表现诱导效应,但高质量浓度Pb2+(144、288 mg/L)处理24 h后,其GSH-Px活性表现为抑制效应.与肝GSH-Px相比,草鱼肝SOD经Pb2+处理后其活性抑制更加明显,表明肝SOD对Pb2+的毒性反应比肝GSH-Px敏感.同时,草鱼经不同质量浓度Pb2+处理12 h后,肝与鳃的MDA含量均比对照组明显增加.上述研究结果表明,水体铅可能通过诱导草鱼肝脏与鳃发生氧化损伤而发挥毒性作用,并进一步损伤机体结构及其生理功能.
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| 关 键 词： | 草鱼 Pb2+ 抗氧化能力 SOD GSH-Px MDA |
Effects of High Concentration Pb2+ on Antioxidant Capability in Juvenile Grass Carp (Ctenopharyngodon idellus) |
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| ZHENG Qing-mei,GUO Jiang-shan,WEN Ru-shu,HAN Chun-yan.Effects of High Concentration Pb2+ on Antioxidant Capability in Juvenile Grass Carp (Ctenopharyngodon idellus)[J].Fisheries Science,2010,29(11). |
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| Authors: | ZHENG Qing-mei GUO Jiang-shan WEN Ru-shu HAN Chun-yan |
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| Institution: | ZHENG Qing-mei1,GUO Jiang-shan2,WEN Ru-shu1,HAN Chun-yan1(1.College of Life Science,Jiaying University,Meizhou 514015,China,2.Meizhou Fisheries Research Institute,Meizhou 514071,China) |
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| Abstract: | Grass carp(Ctenopharyngodon idellus) was exposed to Pb2+ at concentrations of 0,72,144 or 288 mg /L,and activities of glutathion peroxidase(GSH-Px),and dismutase(SOD) and contents of malonyldialdehyde(MDA) in hepatopancrease and gills were determined at 12,24,48 and 96 h after the exposure during the acute toxicity test.Results showed that the activity of GSH-Px in the hepatopan crease was higher in the fish exposed to Pb2+ of different concentrations for 24 h than that in the control group.In the gills,how... |
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| Keywords: | Ctenopharyngodon idellus Pb2 antioxidant capacity SOD GSH-Px MDA |
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