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病毒感染对栉孔扇贝血淋巴中过氧化氢酶和过氧化物酶活力的影响
引用本文:孙虎山,王宜艳,杨静,孙修勤.病毒感染对栉孔扇贝血淋巴中过氧化氢酶和过氧化物酶活力的影响[J].水产科学,2004,23(8):4-6.
作者姓名:孙虎山  王宜艳  杨静  孙修勤
作者单位:1. 烟台师范学院生命科学学院,山东,烟台,264025
2. 国家海洋局第一海洋研究所,山东,青岛,266061
基金项目:国家973计划资助项目(G1999012005),山东省中青年学术骨干项目资助
摘    要:用从病贝体内分离的非包涵体球形病毒感染栉孔扇贝,采用分光光度技术,分别于6,24,72h,测定栉孔扇贝血清和血细胞中过氧化氢酶(CAT)活力和过氧化物酶(POD)活力。结果表明,感染病毒后,血清中CAT活力在6,24,72h时,实验组均高于对照组,6h时差异极显著,24h时差异显著,72h时差异不显著;血细胞中CAT活力在6,24,72h时,实验组均高于对照组,6h和24h时差异极显著,72h时差异不显著。血清和血细胞中POD活力在6h和24h时,实验组均高于对照组,且均差异极显著。说明病毒感染后栉孔扇贝血淋巴中CAT和POD的活力增强。

关 键 词:球形病毒  栉孔扇贝  血淋巴  过氧化氢酶  过氧化物酶  酶活力
文章编号:1003-1111(2004)08-0004-03
修稿时间:2004年1月7日

Effects of Virus on the Activities of Catalase and Peroxidase in Haemolymph of Chlamys farrei
SUN Hu-shan,WANG Yi-yan,YANG Jing,SUN Xiu-qin.Effects of Virus on the Activities of Catalase and Peroxidase in Haemolymph of Chlamys farrei[J].Fisheries Science,2004,23(8):4-6.
Authors:SUN Hu-shan  WANG Yi-yan  YANG Jing  SUN Xiu-qin
Institution:SUN Hu-shan~1,WANG Yi-yan~1,YANG Jing~1,SUN Xiu-qin~2
Abstract:Catalase (CAT) and peroxidase (POD) in the haemocytes and serum of Chlamys farrei were assayed at 6, 24 and 72 h after injection of the non-occluded spherical viruses isolated from the infectious tissues of the scallop collected in the period of massive death in Jiaozhou Bay. The results showed that the CAT activities in serum and haemocyte at 6 h (p<0.01), 24 h (p<0.05) and 72 h (p>05) after injection were significantly higher than those in the controled groups. The POD activities in serum and haemocytes at 6 h and 24 h after injection were significantly higher (p<0.01)than those in the controled groups.
Keywords:spherical viruses  Chlamys farreri  haemolymph  catalase  Peroxidase  activities of enzyme
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