C、N、P比例对2株盐藻生长和生化组成的影响

采用正交设计法研究了C、N、P比例对2株盐藻OUN04和OUN05生长、β-胡萝卜素积累、叶绿素a合成、脂肪酸组成、蛋白质含量的影响。试验结果表明,适当调节C、N、P比例可以促进盐藻OUN04生长、β-胡萝卜素积累、叶绿素a合成,盐藻OUN04最大细胞密度114.2×104个/ml、β-胡萝卜素最大含量100.4 mg/g、叶绿素a最高含量144.6 mg/g分别出现在C、N、P比例为12∶1∶0.05、12∶2∶0.1、12∶1∶0.05的处理组中,且均与对照组差异显著;适当调节C、N、P比例也可以提高盐藻OUN04脂肪酸和蛋白质含量。盐藻OUN05细胞最大密度101.2×104个/ml、β-胡萝卜素最高含量109.2 mg/g、叶绿素a最高含量108.7 mg/g分别出现在C、N、P比例为6∶2∶0.05、6∶0.5∶0.1、12∶2∶0.1的处理组中,且均与对照组差异显著;最大蛋白含量34.41%出现在6∶1∶0.05的处理组中,但与对照组(33.17%)差异不显著。

Effects of Ratio among C, N, and P on Growth and Biochemical Compositions in Two Strains of Alga Dunaliella salina

The effects of ratio among C, N, and P on the growth and biochemical compositions in two strains of alga Dunaliella salina called OUN04, and OUN05 were investigated in an orthogonal design test. The maximum cell density of 114.2×10~4 cell/ml, β-carotene content of 100.4 mg/g and chlorophyll-a content of 144.6 mg/g were detected in the alga OUN04 exposed to the media with the ratios of C∶N∶P=12/1/0.05, 12/2/0.1, and 12/1/0.05, respectively, significantly different from those in the control groups. The maximum content of total fatty acids was found to be 86.20% in the OUN04, significantly higher than that in the control group(81.02%). The alga OUN04 had the maximum protein content of 32.44% when the alga was exposed to the medium with the ratios of C∶N∶P=6/1/0.05, insignificantly different from that in the control group. For the alga OUN05, the maximum cell density of 101.2×10~4 cell/ml, β-carotene content of 109.2 mg/g and chlorophyll-a content of 108.7 mg/g were detected in the treated groups with C∶N∶P=6/2/0.05, 6/0.5/0.1, and 12/2/0.1, respectively, significantly different from those in the control groups. The maximum protein content of 34.41% was observed in the C∶N∶P=6/1/0.05 group of, insignificantly different from that in the control group(33.17%).