| 条斑紫菜eIF4A基因的电子克隆与试验验证 |
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| 引用本文： | 易乐飞,王萍,周向红.条斑紫菜eIF4A基因的电子克隆与试验验证[J].水产科学,2009,28(12). |
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| 作者姓名： | 易乐飞 王萍 周向红 |
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| 作者单位： | 淮海工学院,江苏省海洋生物技术重点实验室,江苏,连云港,222005 |
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| 基金项目： | 江苏省海洋生物技术重点实验室开放课题，淮海工学院自然科学基金资助项目 |
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| 摘 要： | 以拟南芥eIF4A氨基酸序列为信息探针,在条斑紫菜EST(Expressed sequence tag)数据库中找到高度相似的EST,通过人工序列拼接及RT-PCR确认得到了翻译起始因子4A(Eukaryotic translation initiation factors 4A,eIF4A)基因PyeIF4A的cDNA序列.该cDNA序列含有长1278 bp的完整开放阅读框,编码蛋白(PyeIF4A)分子量47.4 kDa,长425 AA.PyeIF4A与小鼠、非洲爪蟾、水稻和拟南芥等多种动植物的eIF4A具有较高的序列一致性,含有eIF4A的保守基序.
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| 关 键 词： | 条斑紫菜 翻译起始因子4A 电子克隆 |
Cilico Cloning and Verification of eIF4A Gene from Laver Porphyra yezoensis |
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| YI Le-fei,WANG Ping,ZHOU Xiang-hong.Cilico Cloning and Verification of eIF4A Gene from Laver Porphyra yezoensis[J].Fisheries Science,2009,28(12). |
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| Authors: | YI Le-fei WANG Ping ZHOU Xiang-hong |
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| Institution: | YI Le-fei,WANG Ping,ZHOU Xiang-hong(Jiangsu Key Laboratory of Marine Biotechnology,Huaihai Institute of Technology,Lianyungang 222005,China) |
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| Abstract: | The silico cloning,verification and characterization of eukaryotic translation initiation factors 4 A(eIF4A)genes was conducted in cDNA of laver Porphyra yezoensis(PyeIF4A).The highly similar P.yezoensis ESTs were obtained by Arabidopsis thaliana eIF4A amino acids sequence as a querying probe from dbEST of GenBank and the putative cDNA sequence of PyeIF4A was assembled.Furthermore,the cDNA of PyeIF4A was isolated by RT-PCR with two primers designed in accordance with the assembled sequence and was found to ... |
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| Keywords: | Porphyra yezoensis eukaryotic translation initiation factors 4A silico cloning |
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