紫外线照射对中华锯齿米虾胚胎发育的影响

自抱卵的中华锯齿米虾雌虾腹部剥离取卵裂期、囊胚期、原肠胚期和无节幼体Ⅰ期的胚胎置于培养皿中,暴露在紫外线下,照射剂量分别为0、1、2、4、8、16 W·s/cm^2,人工振荡培养箱中以速度55 r/min、温度25℃、湿度60%培养。每隔24 h在解剖镜下观察胚胎发育至48 h后进行数据分析,研究紫外线照射剂量对中华锯齿米虾胚胎发育的影响。结果表明,胚胎的存活率对紫外线具有明显的剂量依赖关系。随着紫外线照射剂量的增加,胚胎发育延缓。紫外线暴露后48 h的半数致死量分别为:卵裂期11.5 W·s/cm^2、囊胚期8.6 W·s/cm^2、原肠胚期12.0 W·s/cm^2、无节幼体Ⅰ期14.2 W·s/cm^2。相同的暴露剂量下,暴露时间对胚胎发育也有明显影响。

Effects of Ultraviolet Radiation Intensity on Embryonic Development of Chinese Freshwater Prawn Neocaridina denticulata sinensis

The embryos of Chinese freshwater prawn Neocaridina denticulata sinensis were detached in petri dishes from the abdomen at stages of cleavage,blastula,gastrula and nauplius larvaⅠand exposed to ultraviolet radiation(UV)at doses of 0 W·s/cm^2,1 W·s/cm^2,2 W·s/cm^2,4 W·s/cm^2,8 W·s/cm^2 and 16 W·s/cm^2 in a manual shaking incubator at a speed of 55 r/min,a temperature of 25℃,and a humidity of 60%.The embryonic development was observed under a dissecting scope every 24 hours,and data analysis was performed after 48 h to investigate the effect of UV dose on the embryonic development of Chinese freshwater prawn.The results showed that Chinese freshwater prawn had the survival rate of the embryo in a dose-dependent relationship with UV dose exposure with severely delayed embryo developmen with the increase in UV dose.The median lethal dose at the 48 h was found to be 11.5 W·s/cm^2 in cleavage stage,8.6 W·s/cm^2 in blastula stage,12.0 W·s/cm^2 in gastrula stage,and 14.2 W·s/cm^2 in nauplius I stage.In addition,exposure period showed significant impact on embryonic development.