异型雄性罗氏沼虾遗传多样性的微卫星分析

利用微卫星DNA标记研究3种近亲交配形态型雄性罗氏沼虾遗传多样性,所用虾样均采自国家级(南宁)罗氏沼虾良种场,共150尾成年近亲交配的雄虾,其中蓝色长臂型、橙色长臂型、小个体型各50尾。研究结果表明,3组样品的生长发育差异显著,蓝色长臂型、橙色长臂型、小个体型各组中微卫星位点的总等位基因分别为24、28和22,3组样品所有位点的平均观察杂合度为0.474～0.556。其平均群体内近交系数为-0.0189,说明杂合子过剩,对比分析和群体间分化系数表明,3组雄虾遗传差异明显,最大遗传间距位于蓝色长臂型和小个体型,最小的遗传间距位于蓝色长臂型和橙色长臂型。研究结果将为罗氏沼虾优质父本选育和调控雄性遗传发育提供基础性遗传信息。

Microsatellite Analysis of Genetic Diversity of Male Giant Freshwater Prawn Macrobrachium rosenbergii with Various Morphotypes

Genetic diversity was estimated in 150 inbreeding individuals of three morphotypes blue claw male (BCM), orange claw male (OCM), and small male (SM)] of male giant freshwater prawn Macrobrachium rosenbergii sampled from National (Nanning) shrimp breeding farm, Nanning, Guangxi, using microsatellite markers. Significant differences in growth were observed among all groups, with total alleles of 24 per locus in BCM, 28 in OCM and 22 in SM. The average observed heterozygosities ( H o ) across all loci was ranged from 0.474 to 0.556 in three groups, with mean F IS of -0.0189, indicating that the heterozygosities were excess. The pair-wise comparisons and the F ST values revealed that there was significant genetic differentiation in all groups. The maximal genetic distance was observed between BCM and SM groups, and the minimal one between BCM and OCM groups. The findings provide basic genetic information on selecting high quality giant freshwater prawn and regulating genetic development in male.