还原型谷胱甘肽对大菱鲆生长及抗氧化能力的影响

在基础饲料中添加不同剂量的还原型谷胱甘肽,添加量分别为0、100、200、400、600 mg/kg,投喂初始体质量为(23.08±0.09) g的大菱鲆,8周后测定还原型谷胱甘肽对大菱鲆生长及抗氧化能力的影响。试验结果表明,饲料中还原型谷胱甘肽添加量为200 mg/kg时,大菱鲆的质量增加率和特定生长率显著高于其他组(P<0.05)。饲料中添加还原型谷胱甘肽对大菱鲆肝脏中丙二醛含量、总抗氧化能力、超氧化物歧化酶活力、谷胱甘肽过氧化物酶活力均无显著影响(P>0.05)。随着还原型谷胱甘肽添加量的增加,大菱鲆肝脏中丙二醛含量呈先降后升的趋势,对照组最高,200 mg/kg试验组最低;大菱鲆肝脏中总抗氧化能力、超氧化物歧化酶活力和谷胱甘肽过氧化物酶活力均呈先升后降的趋势,200 mg/kg试验组最高。200 mg/kg试验组和400 mg/kg试验组大菱鲆肝脏中还原型谷胱甘肽含量显著高于对照组(P<0.05)。200 mg/kg试验组大菱鲆肝脏中谷胱甘肽硫转移酶活力和谷胱甘肽还原酶活力显著低于对照组(P<0.05)。根据回归分析,确定大菱鲆饲料中还原型谷胱甘肽的最适添加量为189.70 mg/kg。

Effects of Dietary Reduced Glutathione on Growth and Antioxidation Capacity of Turbot Scophthalmus maximus

An 8-week feeding trial was conducted to evaluate effects of dietary glutathione (GSH) on growth performance and antioxidation capacity of turbot Scophthalmus maximus . The turbot with initial weight of (23.08±0.09) g were fed the basal diet supplemented with 0, 100, 200, 400, and 600 mg/kg reduced glutathione (GSH), respectively, at water temperature of (14.0-18.5)℃. The results showed that weight gain rate and specific growth rate were significantly higher in the turbot in 200 mg/kg dietary GSH group than those in the other groups ( P <0.05). There were no significant differences in the malondialdehyde (MDA) level, total antioxidant capacity (T-AOC), superoxide dismutase (SOD) activity and glutathione peroxidase (GSH-PX) activity in the liver of turbot ( P >0.05). With the increase of the dietary GSH, there was the maximal MDA level in the liver of turbot in the control group, and the minimal in the turbot in the 200 mg/kg GSH group. All the T-AOC, SOD activity and GSH-PX activity in the liver were shown to be a trend of increasing at first and then decreasing, the maximum in the 200 mg/kg dietary GSH group. In the 200 mg/kg and 400 mg/kg dietary GSH groups, the GSH of the liver of turbot was significantly higher than that in the control group ( P <0.05). In the 200 mg/kg dietary GSH group, the GST activity and the GR activity in the liver was significantly lower than that in the control group ( P < 0.05 ). The regression analysis revealed that the appropriate level of dietary GSH was 189.70 mg/kg.