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| 17-β雌二醇对西伯利亚鲟卵黄蛋白原的诱导及检测 | |
| 引用本文： | 庄平,丁建文,侯俊利,张涛,章龙珍,王斌,刘鉴毅,黄晓荣.17-β雌二醇对西伯利亚鲟卵黄蛋白原的诱导及检测[J].海洋渔业,2010,32(3). |
| 作者姓名： | 庄平 丁建文 侯俊利 张涛 章龙珍 王斌 刘鉴毅 黄晓荣 |
| 作者单位： | 1. 中国水产科学研究院东海水产研究所,农业部海洋与河口渔业资源及生态重点开放实验室,上海,200090 2. 华东理工大学生物工程学院,上海,200237 3. 浙江省杭州千岛湖鲟龙科技开发有限公司,杭州,311700 |
| 基金项目： | 农业科技成果转化基金，国家公益性行业(农业)科研专项，国家高技术研究发展计划(863计划项目)，中央级公益性科研院所基本科研业务费专项资金资助项目 |
| 摘 要： | 以17β雌二醇(E2)诱导西伯利亚鲟幼鱼,取其血清样品后利用凝胶过滤层析进行分析,诱导组鱼血清中较对照组的多出一蛋白峰;收集浓缩该峰后经离子交换两步层析法进行分离,成功分离出西伯利亚鲟卵黄蛋白原(Vtg)。分离出的Vtg于7.5%的Native-PAGE凝胶电泳分析,得出其分子量为390 KDa;对Vtg氨基酸组成检测结果显示,谷氨酸、丙氨酸、赖氨酸、天冬氨酸和亮氨酸含量较高,分别为12.25%、10.15%、8.43%、8.37%和8.11%,但甲硫氨酸和组氨酸含量相比较低,分别为1.56%和2.42%。 |
| 关 键 词： | 西伯利亚鲟 卵黄蛋白原 凝胶过滤层析 离子交换层析 |
Induction and determination of Vitellogenin in Acipenser baerii exposed to 17β-estradiol |
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| ZHUANG Ping,DING Jian-wen,HOU Jun-li,ZHANG Tao,ZHANG Long-zhen,WANG Bin,LIU Jian-yi,HUANG Xiao-rong.Induction and determination of Vitellogenin in Acipenser baerii exposed to 17β-estradiol[J].Marine Fisheries,2010,32(3). | |
| Authors: | ZHUANG Ping DING Jian-wen HOU Jun-li ZHANG Tao ZHANG Long-zhen WANG Bin LIU Jian-yi HUANG Xiao-rong |
| Abstract: | Vitellogenin(Vtg) of Acipenser baerii serum was isolated,analysed and determined after being exposed to 17β-estradiol.Results showed that there was another protein peak comparing to the control group after being exposed to 17β-estradiol in Acipenser baerii serum by gel filtration chromatography.Vtg was effectively purified from the serum of Acipenser baerii by two steps of ion-exchange chromatography.The molecular weight of Vtg was 390 KDa through the Native-PAGE gel electrophoresis.Amino acid composition analysis showed that the Vtg was abundant in glutamic acid(12.25%),alanine(10.15%),lysine(8.43%),aspartic acid(8.37%) and leucine(8.11%),but the content of methionine and histamine was lower,1.56% and 2.42% respectively. |
| Keywords: | Acipenser baerii vitellogenin gel filtration chromatography ion-exchange chromatography |
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