| 乙醇-超声法在浮游植物叶绿素a含量测定中的应用 |
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| 引用本文： | 杨洪芳,丁峰元,陈德辉.乙醇-超声法在浮游植物叶绿素a含量测定中的应用[J].海洋渔业,2006,28(4):309-313. |
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| 作者姓名： | 杨洪芳 丁峰元 陈德辉 |
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| 作者单位： | 1. 上海师范大学,生命与环境科学学院,上海,200234
2. 中国水产科学研究院,东海水产研究所,上海,200090 |
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| 摘 要： | 叶绿素测定是生物监测及水质评价中估算藻类生物量的重要指标。以超声波破碎藻细胞,以热乙醇为萃取剂测定浮游植物叶绿素a的含量,并与常规的丙酮-研磨法进行比较对照,结果证实该方法是可行的。其提取时间仅为10m in,因此尤适于快速检测。
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| 关 键 词： | 浮游植物 叶绿素a 乙醇 超声法 丙酮 |
| 文章编号： | 1004-2490（2006）04-0309-05 |
| 收稿时间： | 2006-09-20 |
| 修稿时间： | 2006-09-20 |
Application of ethanol-sonication methods to the determination of phytoplankton chlorophyll-a content |
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| YANG Hong-fang,DING Feng-yuan,CHEN De-hui.Application of ethanol-sonication methods to the determination of phytoplankton chlorophyll-a content[J].Marine Fisheries,2006,28(4):309-313. |
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| Authors: | YANG Hong-fang DING Feng-yuan CHEN De-hui |
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| Institution: | 1. College of Life and Environment Sciences, Shanghai Normal University, Shanghai 200234, China; 2. East China Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Shanghai 200090, China |
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| Abstract: | The determination of chlorophyll is a key index in monitoring water quality. Algal cells were broken up with ultrasonic and chlorophyll-a was extracted with hot ethanol from phytoplankton. This method was compared with a regular method by acetone-rubbing. It was proved that ethanol-sonication could be used normally in the determination of chlorophyll-a, especially in an emergency, because chlorophyll-a could be extracted in only ten minutes. |
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| Keywords: | Phytoplankton chlorophyll-a ethanol sonieation acetone |
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