| 同位素稀释液质联用法测定鱼体中氯丙嗪的残留 |
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| 引用本文： | 于慧娟,王庆贺,戚隽渊,惠芸华,黄冬梅,金高娃,冯兵.同位素稀释液质联用法测定鱼体中氯丙嗪的残留[J].海洋渔业,2011,33(1):83-90. |
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| 作者姓名： | 于慧娟 王庆贺 戚隽渊 惠芸华 黄冬梅 金高娃 冯兵 |
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| 作者单位： | 1. 中国水产科学研究院东海水产研究所,上海,200090
2. 上海水产研究所,上海,200433 |
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| 基金项目： | 农业部行业标准制、修订 |
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| 摘 要： | 建立了高效液相色谱-串联质谱(HPLC/MS/MS)分析鱼体中氯丙嗪残留的方法.样品用乙腈提取,MCX固相萃取柱净化,氨化甲醇洗脱.在正离子模式下以电喷雾串联质谱仪进行测定,采用内标法定量,氘代氯丙嗪为内标物.以欧洲鳗鲡、鳜鱼和大口黑鲈为研究对象,在1.0、5.0、25.0μg/kg添加水平下,方法平均回收率为90.1...
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| 关 键 词： | 氯丙嗪 同位素稀释 高效液相色谱-串联质谱 欧洲鳗鲡 鳜鱼 大口黑鲈 |
Determination of Chlorpromazine residue in fishies by isotope dilution liquid chromatography-tandem mass spectrometry |
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| YU Hui-juan,WANG Qin-he,Qi Jun-yuan,HUI Yun-hua,HUANG Dong-mei,JIN Gao-wa,FENG Bing.Determination of Chlorpromazine residue in fishies by isotope dilution liquid chromatography-tandem mass spectrometry[J].Marine Fisheries,2011,33(1):83-90. |
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| Authors: | YU Hui-juan WANG Qin-he Qi Jun-yuan HUI Yun-hua HUANG Dong-mei JIN Gao-wa FENG Bing |
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| Abstract: | A method to detect chlorpromazine residues in fish products was developed by using high performance liquid chromatography-tandem mass spectrometry(HPLC/ MS/MS).Chlorpromazine was extracted with acetonitrile from the test tissue.The extract was cleaned up and concentrated on an Oasis MCX cartridge,eluted by basic methanol and then analyzed by HPLC-MS/MS.The quantification was performed with internal standard method,with D6-Chlorpromazine as internal standard.The average recoveries were 90.1%-95.1%(Intraday) in the spiked range of 1-25 μg/kg in fish.The relative standard deviations were 2.5%-5.7%(Intraday).The limits of quantification(S /N >10) were 1.0 μg/kg and the limits of detection(S /N >3) were 0.5 μg/kg.Results indicated that the method used was easier,faster and more accurate. |
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| Keywords: | Chlorpromazine isotope dilution HPLC/MS/MS Anguilla Anguilla Siniperca chuatsi Micropterus salmoides |
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