卵形鲳鲹消化酶活性的研究Ⅳ养殖水温和酶反应温度对幼鱼酶活性的影响

比较研究了不同的养殖水温和酶反应温度对卵形鲳够幼鱼(体重为0.82±0.15 g)消化酶活性的影响.在第一组试验,将卵形鲳够幼鱼分别在19℃、22℃、25℃、28℃和31℃各温度下养殖7 d,然后在相同的酶反应温度下测定其消化酶活性;第二组试验,将卵形鲳够幼鱼在25～26℃驯养7 d,然后分别在15℃、20℃、25℃、...

On digestive enzyme activity in Trachinotus ovatus Ⅳ Effects of cultural and reactive temperatures on major enzyme activity in juveniles

The variation of activity of three digestive enzymes in juvenile Trachinotus ovatus with body weight of 0.82±0.15 g was investigated under different cultural and reactive temperatures.In the first group,juveniles were acclimated at five different water temperatures(19 ℃,22 ℃,25 ℃,28 ℃ and 31 ℃) for 7 days,respectively.After starvation for 24 hours,fish samples were seleted randomly and enzyme activity were dissected at the same reactive temperature.In the second group,juveniles were acclimated at the natural temperatures of 25-26℃ for 7 days.Enzyme activity were measured at eight different reactive temperatures(15 ℃,20 ℃,25 ℃,30 ℃,35 ℃,40 ℃,45 ℃ and 50 ℃).Results showed that the enzyme activity increased with the cultural temperatures increasing and then decreased in the range from 19 to 31 ℃.The optimum cultural temperatures for protease,amylase and lipase were 28℃,25℃ and 22℃,respectively.The activity of three digestive enzymes increased with the reactive temperatures increasing and then decreased in the range from 15 to 50 ℃.The optimum reactive temperatures for protease,amylase and lipase were 40 ℃,35 ℃and 40 ℃,respectively.The optimum reactive temperatures were obviously higher than the cultural temperatures.