基于线粒体D-loop基因序列研究我国5种虾虎鱼类的系统进化关系

利用PCR技术扩增河川沙塘鳢(Odontobutis potamophila)、鸭绿沙塘鳢(Odontobutis yaluensis)、中华沙塘鳢(Odontobutis sinensis)、葛氏鲈塘鳢(Perccottus glenii)及尖头塘鳢(Eleotris oxycephala)的线粒体D-loop区并测序,获得河川沙塘鳢、鸭绿沙塘鳢、中华沙塘鳢、葛氏鲈塘鳢及尖头塘鳢线粒体D-loop区全序列分别为853bp、853bp、1028bp、852bp、848bp,结合GenBank中平头沙塘鳢(Odontobutis platycephala)、刺盖塘鳢(Eleotris acanthopoma)、黑体塘鳢(Eleotris melanosoma)、褐塘鳢(Eleotris fusca)、中华乌塘鳢(Bostrychussinensis)、云斑尖塘鳢(Oxyeleotris marmorata)、溪鳢(Rhyacichthys aspro))等7种虾虎鱼类同源的D-loop区序列,分析了这12种虾虎鱼类的系统发育关系。所使用的同源序列长度为832～846bp,其中保守位点452个,变异位点405个,简约信息位点258个,单态位点145个,转换/颠换平均值为0.8。基于Kimura2-parameter模型的12种虾虎鱼类遗传距离范围为0.043～0.312,在Kimura2-parameter模型构建的邻接树和非加权配对法系统树中,河川沙塘鳢、鸭绿沙塘鳢、中华沙塘鳢、平头沙塘鳢聚为一大支,刺盖塘鳢、黑体塘鳢、褐塘鳢、尖头塘鳢、溪鳢、葛氏鲈塘鳢聚为另一支。

Phylogenetic relationships of 5 gobioidei species based on sequences of mitochondrial DNA D-loop

To investigate the molecular phylogeny relationship of Gobioidei species fish,D-loop of five species fish(Odontobutis potamophila,Odontobutis yaluensis,Odontobutis sinensis,Perccottus glenii and Eleotris oxycephala) were cloned by PCR methods.PCR amplification products of complete D-loop gene fragments from five species fish were 853 bp,853 bp,1 028 bp,852 bp and 848 bp,respectively.Together with homologous D-loop sequences of seven species(Odontobutis platycephala,Eleotris acanthopoma,Eleotris melanosoma,Eleotris fusca,Bostrychus sinensis,Oxyeleotris marmorata and Rhyacichthys aspro) from GenBank,D-loop sequences of twelve species fish were analyzed by MEGA 4.0 software.The results showed that mitochondrial DNA D-loop sequences were 832-846 bp,of which 452 sites were conserved,405 sites were variable,258 sites were parsimony informative,145 sites were singleton sites and the average transitionsal /transversional was 0.8.Kimura 2-parameter model-based pairwise genetic distances within twelve Gobioidei species were analyzed,and each putative species ranged from 0.043-0.312.And based on phylogenetic trees(NJ) and(UPGMA) analysis,the results showed that O.potamophila,O.yaluensis,O.sinensis and O.platycephala belonged to the same branch,E.acanthopoma,E.melanosoma,E.fusca,E.oxycephala,R.aspro and P.glenii belonged to another branch.