我国五个青蛤地理群体遗传变异的RAPD分析

本文利用RAPD方法对我国浙江、江苏、辽宁、天津和福建沿海青蛤地理群体的遗传变异和遗传结构进行了分析。从60条随机引物中,筛选出其中12条扩增效果好、条带清晰的引物进行扩增。结果表明,青蛤遗传多样性较丰富,五个群体的多态度在0.249～0.307之间,平均为0.278,按递减依次为江苏>福建>辽宁>浙江>天津;经分子方差分析(AMOVA),10.69%的遗传变异来自于群体间,89.31%来自于群体内,遗传分化除辽宁与江苏、天津群体间不显著外,其它群体间分化显著;根据遗传距离,用UPGMA法进行聚类分析表明,浙江群体与江苏群体,辽宁群体与天津群体分别先聚在一起,最后与福建群体聚类;S11、S424、S228、S4254个引物可以区分这五个青蛤地理种群,作为群体特征标记。

Analysis of genetic variations of five geographical populations in Cyclina sinensis (Gmelin) of China by RAPD

RAPD was conducted to analyse the genetic variations and genetic structure of five geographical populations in Cyclina sinensis from Zhejiang, Jiangsu, Liaoning, Tianjin and Fujian Provinces. Twelve polymorphic random primers were selected from 60 to amplify. The results indicated that the genetic diversity of Cyclina sinensis was rich, genetic diversity in five populations were ranged from 0.249 to 0.307 with an average of 0.278, and the genetic diversity of Jiangsu, Fujian, Liaoning, Zhejiang and Tianjin populations had in turn less than the one before it. The results of analysis of molecular variance(AMOVA) showed that there was significant genetic variations between populations except between Liaoning and Jiangsu populations, Liaoning and Tianjin populations. First Zhejiang and Jangsu populations clustered, then Liaoning and Tianjin populations clustered, and finally they clustered with Fujian population by UPGMA method according to their genetic distances. The differences of finger printings obtained by primers S11, S424 , S228 and S425 were obviously enough to be used as molecular markers to distinguish five populations.