细鳞鲑对谷氨酸行为趋向的反应阈值

为了筛选细鳞鲑(Brachymystax lenok)的氨基酸类诱食剂,通过Y型迷宫实验研究了细鳞鲑幼鱼对不同浓度谷氨酸(glutamic acid)刺激液的行为趋向及反应阈值。依据行为反应评分和偏好度分析结果,细鳞鲑幼鱼对1×10^-5、1×10^-6、1×10^-7、1×10^-8、1×10^-9 mol·L^-1的谷氨酸刺激液的相对偏好度分别为0.396、0.436、0.381、3.801、1.142,表明细鳞鲑幼鱼对1×10^-5、1×10^-6、1×10^-7 mol·L^-1谷氨酸有负趋向反应,对1×10^-8、1×10^-9 mol·L^-1谷氨酸有正趋向性,其中对1×10^-8 mol·L^-1谷氨酸的趋向性与对照组有显著性差异(P<0.05),其嗅觉行为趋向的反应阈值为0.7×10^-8 mol·L^-1。结果表明,谷氨酸可作为细鳞鲑潜在的氨基酸类诱食剂。

Response threshold of Brachymystax lenok to glutamic acid stimulus

At present, the distribution area of Brachymystax lenok is gradually reduced and the biomass has dropped sharply due to various human factors in China. In order to protect and recover the resources, the conservation of natural resources and artificial breeding was carried out. In fish culture, palatability is an important prerequisite for the compound feed to be quickly ingested and absorbed by fish. Therefore, it is necessary to add an attractant substance to promote feeding and to improve the feeding efficiency. Amino acids can induce feeding-related olfactory behavior and are considered to be one of the most effective fish attractant compounds. Studies have shown that both glutamic acid and proline can effectively trigger the olfactory imprinting of Onsuhynchus nerka. It is indicated that the approach to amino acids stimulus in fish may be endogenous, which also confirms the mechanism of amino acids as attractants. However, the behavior response of Brachymystax lenok to amino acids is still unclear. In this paper, the Y maze experiments were conducted to test the behavioral responses of Brachymystax lenok to the glutamic acid stimulus with a concentration of 1×10^-5 to 1×10^-9 mol·L^-1. According to the results of behavioral response scores and preference analysis, the relative preference of fish to 1×10^-5, 1×10^-6, 1×10^-7, 1×10^-8, 1×10^-9 mol·L^-1was 0.396, 0.436, 0.381, 3.801, 1.142, respectively. It was indicated that the fish had a negative tendency to 1×10^-5, 1×10^-6, 1×10^-7 mol·L^-1glutamic acid, but had a positive trend toward 1×10^-8, 1×10^-9 mol·L^-1glutamic acid, and there was a significant difference in 1×10^-8 mol·L^-1test group(P<0.05). The response threshold of behavioral response was 0.7×10^-8 mol·L^-1. The results indicate that glutamic acid can be used as a potential amino acid attractant for young Brachymystax lenok.