半滑舌鳎生长性状的微卫星标记筛选

结合分离群体标记关联分析法与随机选择群体标记关联分析法,利用69个微卫星位点,检测半滑舌鳎(Cynoglossus semilaevis)半同胞家系的极端大群体和极端小群体,筛选出12个与全长、体高、体重显著或极显著相关的微卫星位点。验证群体的平均等位基因数为5.167个,群体平均有效等位基因数为3.319,平均观测杂合度为0.516,期望杂合度0.670,多态信息含量0.621。分析结果显示:在雌性群体中,微卫星位点csou4、cyse123、ghrh-ssr、cyse72、hncyse160与全长显著相关,ghrh-ssr与体高显著相关,csou4、ghrh-ssr、cyse72、hncyse160与体重相关;在雄性群体中,微卫星位点hncyse129、hncyse160、sox9-1与全长相关,csou34、cyse22、cyse123、hncse67、sox9-1与体高显著相关,未发现与体重相关位点。

Correlation analysis between growth-related traits and microsatellite markers in half smooth tongue sole ( Cynoglossus semilaevis)

69 microsatellite loci were selected to amplify the genomic DNA of cultured stock of half smooth tongue sole(Cynoglossus semilaevis).12 microsatellite loci showing significant difference(P<0.05) were used in genotyping individuals,and the association between genotypes and growth traits was examined.The results showed that a total of 62 alleles were found in 12 loci,with an average of 5.167,and the average effective number of alleles(Ae),observed heterozygosity(Ho),expected heterozygosity(He) and mean polymorphic information content(PIC) was 3.319,0.516,0.670 and 0.621.The results suggested that csou4,cyse123,ghrh-ssr,cyse72 and hncyse160 in female and hncyse129,hncyse160,sox9-1 in male were associated with body length;Ghrh-ssr in female,and csou34,cyse22,cyse123,hncse67,sox9-1 in male were associated with body height.