白斑综合征病毒(WSSV)在拟穴青蟹体内增殖的研究

拟穴青蟹(Scylla paramamosain)因肉质鲜美、生长快速而成为我国主要的海水养殖经济蟹类,但是随着养殖规模的不断扩大,病害问题也越发严重。本研究通过流行病学调查和定量PCR跟踪检测的方法,研究了拟穴青蟹对白斑综合征病毒(WSSV)的易感性和WSSV在拟穴青蟹体内的增殖情况。结果表明,拟穴青蟹是WSSV的自然宿主,自然携带率为8.47%。WSSV可通过口服途径感染拟穴青蟹,并在拟穴青蟹体内快速增殖,注射感染5 d后,病毒量达到感染1d时的1.1×109倍,当病毒累积到一定量后即可致死拟穴青蟹。研究表明,拟穴青蟹是WSSV的天然宿主,在虾蟹混养过程中可以通过摄食WSSV感染虾而带毒或发病,并因此成为WSSV传播媒介,从而对虾蟹混养条件下WSSV的防治效果产生重要影响。

On proliferation of white spot syndrome virus (WSSV) within Green Mud Crab (Scylla paramamosain) by real time PCR

Diseases of Scylla paramamosain,a main culture species in sea waters of China are increasing with the growing of culture areas and density.There are still no studies on the susceptibility of S.paramamosain to WSSV and how WSSV proliferates within mud crab.The investigation showed that there were about 8.47% of green mud crabs carried WSSV in natural sea waters in Hainan Province.Subsequently,studies were conducted on green mud crab by oral administration and muscle injection with WSSV.Results demonstrated that green mud crab could be infected with WSSV by oral administration with diseased shrimp,and WSSV could proliferate very quickly within green mud crab.With the number of WSSV increasing,the mortality of crab was on the increase.Results suggested that S.paramamosain was a natural host of WSSV,which could be infected with WSSV by consuming diseased shrimp and become a medium of WSSV in a polyculture system of shrimp and crab.