黄连素对3种水产动物致病菌的抑制作用研究

文章研究了黄连素对副溶血弧菌（Vibrio parahaemolyticus）、溶藻弧菌（V.alginolyticus）和嗜水气单胞菌（Aeromonas hydrophila）等3种致病菌的最小抑菌浓度（MIC）和最小杀菌浓度（MBC）,检测了黄连素对病原菌菌体形态和生长曲线的影响,并以此解析黄连素对这3种致病菌的抑制特点和抑菌机制.结果显示,黄连素对副溶血弧菌、溶藻弧菌、嗜水气单胞菌的MIC和MBC分别为0.391 mg·mL^-1和0.391 mg·mL^-1、0.391 mg·mL^-1和0.781 mg·mL^-1、0.781 mg·mL^-1和1.563 mg·mL^-1,说明黄连素对3种致病菌均有明显的抑制作用,其中对副溶血弧菌的抑制效果最佳.同步结果显示,1.00 mg·mL^-1黄连素不仅会严重破坏致病菌的菌体形态,而且使致病菌的迟缓期延长2～7h,这暗示黄连素可能通过影响致病菌的细胞结构和生长繁殖来达到抑菌的效果.由此可见,黄连素在防治水产类细菌性疾病上有很好的应用潜力.

Antibacterial effects of Berberine on three aquatic pathogens in vitro

In order to reveal antibacterial effects of Berberine on three aquatic pathogens and its mechanisms, we determined the minimal inhibitory concentration/the minimal bactericidal concentration （MIC/MBC） of Berberine on Vibrio parahaemolyticus, V. alginolyticus and Aeromonas hydrophila, as well as the effects of Berberine on the cell morphology and the cell growth curve of three bacteria. The results show that the MIC and MBC of Berberine on V. parahaemolyticus, V. alginolyticus and A. hydrophila are 0. 391 mg . mL^-1 and 0. 391 mg-mL^-1 , 0. 391 mg-mL^-1 and 0. 781 mg.mL^-1 , 0. 781 mg.mL^-1 and 1. 563 mg.mL^-1 , respectively. These results indicate that Berberine has significant antibacterial effects on tested bacteria, especially its inhibitory effect on V. parahaemolyticus. Besides, 1.00 mg.mL 1 of Berberine can seriously destroy the cell morphology of pathogens, and extend the lag phase of pathogens to 2 - 7 h, which suggests that the antibacterial effects of Berberine might be achieved through affecting the cell structure and growth of pathogens. In conclusion, Berberine is a potential drug against aquatic bacterial diseases.