沼泽红假单胞菌对波吉卵囊藻常温保存效果的影响

波吉卵囊藻（Oocystis borgei）是亚热带对虾高位池养殖水体常见的优势种,对于稳定池塘生态结构,保持良好水体环境具有重要的意义.研究常温下波吉卵囊藻的保存效果有利于波吉卵囊藻的简易保存和虾类养殖的直接投入使用,以便推动水产养殖行业的微藻生态调控.在25℃、30℃和35℃下研究了不同沼泽红假单胞菌（Rhodopseudomonas palustris）浓度对波吉卵囊藻保存效果的影响.结果显示,在25℃、30℃和35℃下波吉卵囊藻保存的成活率、叶绿素a（Chl-a）质量浓度,以及恢复培养后的比增长率和ρ（Chl-a）与沼泽红假单胞菌添加浓度呈正相关,1.94×10^6个·mL-为最佳浓度;25℃为最适保存温度,但是在30℃下保存后恢复生长时发现比增长率要比25℃下保存的高（0.432）.因此,25℃下添加沼泽红假单胞菌浓度为1.94×10^6个·mL^-1时波吉卵囊藻的保存效果最佳,成活率达到87.5％,p（Chl-a）为31.14 μg·L^-1,经恢复培养后比增长率为0.424,p（Chl-a）为55.94 μg·L^-1.

Effect of Rhodopseudomonas palustris on the storage of Oocystis borgei at normal temperature

Oocystis borgei is dominant algae in subtropical prawn farming, which plays an important role in stabling pool ecological structure and regulating water quality. Study on the effect of 0. borgei at normal temperature can promote simple storage and the application of 0. borgei into shrimp and prawn culture, so as to promote technology of microalgae ecological nlanagement. We studied the effect of Rhodopseudomonas palustris with different concentration on the storage of 0. borgei at 25 ℃ , 30 ℃ aml 35 ℃. The results show that, in each temperature the survival rate and chlorophyll a content of 0. borgei are positively correlated with the concentration of R. palustris, as well as its specific growth rate and chlorophyll a content after re-culturing. The best concentration of R. palustris is 1.94 x 106cells.mL-1 , and the optimal temperature for 0. borgei storage is 25 ℃, while the specific growth rate of 0. borgei after reculturing at 30 ℃ （0. 432） is higher than that of at 25 ℃. To sum up, when 1.94 ×10^6cells.mL . R. palustris added at 25 ℃, we may get the optimal 0. borgei storage with the survival rate 87.5% and the chlorophyll a content 31.14 μg·L^-1, and a good storage of 0. borgei after re-culturing with the specific growth rate 0. 424 and the chlorophyll a 55.94 ixg.L^-l.