编码美洲螯龙虾肽聚糖识别蛋白（PGRP）cDNA的电子克隆及其生物信息学分析

利用电子克隆方法，获得编码美洲螯龙虾（Homarus americanus）肽聚糖识别蛋白（peptidoglycan recognition protein , PGRP）的cDNA序列及相应的氨基酸序列；采用生物信息学方法对其进行基本理化性质、亲疏水性、信号肽、二级结构、细胞定位、三级结构等的分析。结果获得一段长920 bp的编码美洲螯龙虾PGRP的cDNA序列，包含一个长834 bp的开放阅读框，编码277个氨基酸；编码蛋白是含有信号肽的分泌蛋白，含有1个C端PGRP结构域，表明是一种PGRP；分析结果显示该蛋白与黑腹果蝇（Drosophila melanogaster）短型PGRP-SC2和PGRP-SD比较相似。

In silico cloning and bioinformatics analysis of the cDNA encoding a PGRP in Homarus americanus

We obtained a cDNA sequence that encoding a putative peptidoglycan recognition protein （PGRP） of American lobster, Homarus americanus, by in silicon cloning in this study; and analyzed the deduced protein by bioinformatics methods in terms of its general physical and chemical properties, hydrophobicity, signal peptide, secondary / tertiary structure and localization sites in cells. The results show that the obtained 920 bp long cDNA contains an open reading frame （834 bp） which encoding a protein with 277 amino acids. The protein has a signal peptide in its N terminal, a PGRP domain in its C terminal, which means that the protein maybe is a secretory protein, short type PGRP. Phylogeny analysis indicates that the protein is more like the PGRP-SC2 and PGRP-SD in Drosophila melanogaster.